전통 발효 식품에서 분리한 신규 젖산균의 프로바이오틱스특성 및 생리활성 평가

This study evaluated the characteristics of lactic acid bacteria (LAB) isolated from kkakdugi for its use as a probiotic. In addition, the possibility of using it as a material for promoting antioxidant activity and skin functionality was evaluated. To verify the feasibility of LAB as probiotics, their survival rates in artificial gastric juice and artificial bile were evaluated. In artificial gastric juice, the average number of probiotics was maintained at 5.3×10⁹ colony-forming units (CFU)/mL, showing a survival rate of about 99%. In artificial bile, the average number of probiotics was maintained at 1.2×10⁹ CFU/mL, showing a survival rate of about 95%. The survival rate indicated their ability to reach the target site to exert their effects. In addition, autoaggregation and cell surface hydrophobicity experiments were conducted to indirectly confirm their ability to adhere to the gastrointestinal tract surface. The autoaggregation rate of all LAB strains increased over time. Specifically, L. plantarum K1-9 and L. brevis K2-9 strains showed high hydrophobicity. LAB culture supernatants were used to evaluate antioxidant activity, antidiabetic activity, and skin functionality, such as the effects on skin wrinkles, whitening, and astringent effects. The results showed that, the LAB isolated from kkakdugi had high antioxidant activity. In addition, increasing the concentration of the LAB culture supernatant is expected to have positive effects on skin wrinkles and whitening. Therefore, it is believed that these characteristics of the LAB isolated from kkakdugi ensure that they have sufficient potential to be utilized as an intestinal probiotic and functional cosmetic materials.