제비고깔(Delphinium grandiflorum)꽃 추출물의 항산화, 항염증,피부 주름 생성 억제 및 모공축소 효과

Several phenolic compounds obtained from plants exert good biological activities. Delphinium grandiflorum flowers have been used for the prevention or treatment of cancer and osteoporosis. This study examines the antioxidantive and biological enzyme activities of phenolic compounds present in Delphinium grandiflorum flower extracts. Total phenol contents were evaluated using the Folin-Ciocalteu method. Antioxidant activities were determined by measuring DPPH, antioxidant protection factor, ABTS+, and TBARS. We further evaluated inhibitory activity against hyaluronidase, elastase, collagenase and astringent effect. DPPH scavenging activity was determined to be 90.07% and 100% and the ABTS radical scavenging activity was 99.54% and 100% in water and ethanol extract containing 100 μg/mL phenols, respectively. The PF activity confirmed high antioxidant effects at 1.26 PF and 1.27 PF, whereas the TBARs inhibitory activity was 24.05% and 69.55%, in water and ethanol extract, respectively. Examining the inhibitory effects of elastase and collagenase for wrinkle improvement revealed an inhibition rate of 41.21% and 82.58%, respectively, at a phenolics concentration of 400 μg/mL in 70% ethanol extract. Hyaluronidase is known to induce an inflammatory effect. Hyaluronidase inhibitory rates of 24.52% and 16.81% were obtained at 400 μg/mL phenolics concentrations of water and ethanol extract, respectively. The astringent effect showed an activity of 44.82% at a concentration of 400 μg/mL phenolics in the 70% ethanol extract. These results indicate the potential of the DGF extract to be applied as a functional cosmetic and beauty food resource imparting antioxidant, anti-inflammatory, anti-wrinkle and pore-reducing effects.