Abstract
Glycosidases play a pivotal role in processing of various glycoproteins and glycolipids. It is well known that glycosidases are also involved in a variety of degenerative metabolic disorders such as cancer and AIDS. In order to develop potent α-glucosidase inhibitors, we first screened 2,2-diphenyl-1-picrylhydrazyl hydrate as a candidate. 2,2-Diphenyl-1-picrylhydrazyl hydrate was found to inhibit α- and β-glucosidases as well as α- and β-mannosidases. It was also shown to be a non-competitive inhibitor of yeast α-glucosidase with a Ki value of 1.1×10-6 M. Taken together, we anticipate that 2,2-diphenyl-1-picrylhydrazyl hydrate may be a potent inhibitor for some incurable metabolic disorders including AIDS.
Original language | English |
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Pages (from-to) | 727-728 |
Number of pages | 2 |
Journal | Biological and Pharmaceutical Bulletin |
Volume | 24 |
Issue number | 6 |
DOIs | |
State | Published - 2001 |
Keywords
- α-glucosidase inhibitor
- 2,2-Diphenyl-1-picrylhydrazyl hydrate
- Enzyme kinetics
- Non-competitive inhibition