A β-secretase (BACE1)-inhibiting C-methylrotenoid induced by yeast elicitation in Abronia nana suspension cultures

Sang In Kim; Eun Ju Yang; Se Hoon Park; Chang Kil Kim; Kyung Sik Song

doi:10.1007/s12010-014-0762-0

A β-secretase (BACE1)-inhibiting C-methylrotenoid induced by yeast elicitation in Abronia nana suspension cultures

Sang In Kim
, Eun Ju Yang
, Se Hoon Park
, Chang Kil Kim
, Kyung Sik Song

Department of Horticultural Science

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Suspension cultures of Abronia nana were established to produce C-methylisoflavones. Treatment of the A. nana cultures with yeast elicitor induced boeravinone E (1), with maximum induction at 24 h after elicitor treatment. Of the biotic and abiotic elicitors tested, yeast extract gave the strongest induction of 1. The IC₅₀ value of 1 against β-secretase (β-amyloid cleaving enzyme-1) was 5.57 μM. Other proteases such as trypsin, chymotrypsin, and elastase were not inhibited by concentrations up to 1.0 mM, indicating that inhibition of β-secretase was specific. 1 was noncompetitive in Dixon plot, and Ki value was 3.79 μM.

Original language	English
Pages (from-to)	3529-3537
Number of pages	9
Journal	Applied Biochemistry and Biotechnology
Volume	172
Issue number	7
DOIs	https://doi.org/10.1007/s12010-014-0762-0
State	Published - Apr 2014

Keywords

Abronia nana
Boeravinone E
C-methylrotenoid
Suspension cultures
Yeast elicitation
β-secretase inhibitor

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10.1007/s12010-014-0762-0

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title = "A β-secretase (BACE1)-inhibiting C-methylrotenoid induced by yeast elicitation in Abronia nana suspension cultures",

abstract = "Suspension cultures of Abronia nana were established to produce C-methylisoflavones. Treatment of the A. nana cultures with yeast elicitor induced boeravinone E (1), with maximum induction at 24 h after elicitor treatment. Of the biotic and abiotic elicitors tested, yeast extract gave the strongest induction of 1. The IC50 value of 1 against β-secretase (β-amyloid cleaving enzyme-1) was 5.57 μM. Other proteases such as trypsin, chymotrypsin, and elastase were not inhibited by concentrations up to 1.0 mM, indicating that inhibition of β-secretase was specific. 1 was noncompetitive in Dixon plot, and Ki value was 3.79 μM.",

keywords = "Abronia nana, Boeravinone E, C-methylrotenoid, Suspension cultures, Yeast elicitation, β-secretase inhibitor",

author = "Kim, \{Sang In\} and Yang, \{Eun Ju\} and Park, \{Se Hoon\} and Kim, \{Chang Kil\} and Song, \{Kyung Sik\}",

year = "2014",

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doi = "10.1007/s12010-014-0762-0",

language = "English",

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journal = "Applied Biochemistry and Biotechnology",

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TY - JOUR

T1 - A β-secretase (BACE1)-inhibiting C-methylrotenoid induced by yeast elicitation in Abronia nana suspension cultures

AU - Kim, Sang In

AU - Yang, Eun Ju

AU - Park, Se Hoon

AU - Kim, Chang Kil

AU - Song, Kyung Sik

PY - 2014/4

Y1 - 2014/4

N2 - Suspension cultures of Abronia nana were established to produce C-methylisoflavones. Treatment of the A. nana cultures with yeast elicitor induced boeravinone E (1), with maximum induction at 24 h after elicitor treatment. Of the biotic and abiotic elicitors tested, yeast extract gave the strongest induction of 1. The IC50 value of 1 against β-secretase (β-amyloid cleaving enzyme-1) was 5.57 μM. Other proteases such as trypsin, chymotrypsin, and elastase were not inhibited by concentrations up to 1.0 mM, indicating that inhibition of β-secretase was specific. 1 was noncompetitive in Dixon plot, and Ki value was 3.79 μM.

AB - Suspension cultures of Abronia nana were established to produce C-methylisoflavones. Treatment of the A. nana cultures with yeast elicitor induced boeravinone E (1), with maximum induction at 24 h after elicitor treatment. Of the biotic and abiotic elicitors tested, yeast extract gave the strongest induction of 1. The IC50 value of 1 against β-secretase (β-amyloid cleaving enzyme-1) was 5.57 μM. Other proteases such as trypsin, chymotrypsin, and elastase were not inhibited by concentrations up to 1.0 mM, indicating that inhibition of β-secretase was specific. 1 was noncompetitive in Dixon plot, and Ki value was 3.79 μM.

KW - Abronia nana

KW - Boeravinone E

KW - C-methylrotenoid

KW - Suspension cultures

KW - Yeast elicitation

KW - β-secretase inhibitor

UR - https://www.scopus.com/pages/publications/84899410039

U2 - 10.1007/s12010-014-0762-0

DO - 10.1007/s12010-014-0762-0

M3 - Article

C2 - 24557951

AN - SCOPUS:84899410039

SN - 0273-2289

VL - 172

SP - 3529

EP - 3537

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 7

ER -

A β-secretase (BACE1)-inhibiting C-methylrotenoid induced by yeast elicitation in Abronia nana suspension cultures

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