A carbon dot-hemoglobin complex-based biosensor for cholesterol detection

Trang Thi Bui; Soo Young Park

doi:10.1039/c6gc00507a

A carbon dot-hemoglobin complex-based biosensor for cholesterol detection

Trang Thi Bui, Soo Young Park

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor. This optical sensor detects cholesterol through fluorescence enhancement of CD, which is normally quenched via π-π interactions between CD and Hb in the CD/Hb complex. CD is released from the CD/Hb complex because hydrophobic interactions between Hb and cholesterol are more favorable than π-π interactions between CD and Hb. The CD/Hb complex enabled selective detection of cholesterol within a linear range from 0 to 800 μM, with a limit of detection of 56 μM and a response time of ≤5 minutes in human blood plasma. Compared with other cholesterol sensors, a CD/Hb complex-based biosensor is simple, highly sensitive, selective, rapid, eco-friendly, and cost-effective for cholesterol detection in both biological and environmental samples.

Original language	English
Pages (from-to)	4245-4253
Number of pages	9
Journal	Green Chemistry
Volume	18
Issue number	15
DOIs	https://doi.org/10.1039/c6gc00507a
State	Published - 2016

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abstract = "In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor. This optical sensor detects cholesterol through fluorescence enhancement of CD, which is normally quenched via π-π interactions between CD and Hb in the CD/Hb complex. CD is released from the CD/Hb complex because hydrophobic interactions between Hb and cholesterol are more favorable than π-π interactions between CD and Hb. The CD/Hb complex enabled selective detection of cholesterol within a linear range from 0 to 800 μM, with a limit of detection of 56 μM and a response time of ≤5 minutes in human blood plasma. Compared with other cholesterol sensors, a CD/Hb complex-based biosensor is simple, highly sensitive, selective, rapid, eco-friendly, and cost-effective for cholesterol detection in both biological and environmental samples.",

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AB - In the present study, a carbon dot/hemoglobin (CD/Hb) complex is used as a bio-receptor in an optical cholesterol biosensor. This optical sensor detects cholesterol through fluorescence enhancement of CD, which is normally quenched via π-π interactions between CD and Hb in the CD/Hb complex. CD is released from the CD/Hb complex because hydrophobic interactions between Hb and cholesterol are more favorable than π-π interactions between CD and Hb. The CD/Hb complex enabled selective detection of cholesterol within a linear range from 0 to 800 μM, with a limit of detection of 56 μM and a response time of ≤5 minutes in human blood plasma. Compared with other cholesterol sensors, a CD/Hb complex-based biosensor is simple, highly sensitive, selective, rapid, eco-friendly, and cost-effective for cholesterol detection in both biological and environmental samples.

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A carbon dot-hemoglobin complex-based biosensor for cholesterol detection

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