A diazotrophic, indole-3-acetic acid-producing endophyte from wild cottonwood

Gang Xin, Gengyun Zhang, Jun Won Kang, James T. Staley, Sharon L. Doty

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67 Scopus citations

Abstract

An endophytic bacterium, wild poplar strain B (WPB), isolated from stems of wild cottonwood (Populus trichocarpa) was identified to Burkholderia vietnamiensis by analyzing the recA and rDNA genes. Phylogenetic analysis of the nifHDK cluster indicates that the WPB isolate shares high sequence similarity with known B. vietnamiensis strains. The nitrogenase activity of WPB was determined by a 15N2 incorporation assay and an acetylene reduction assay. WPB was also monitored for production of indole-3-acetic acid (IAA), a phytohormone which can promote plant growth, when incubated with L-tryptophan. In addition, its plant growth promotion capacity was assessed by inoculating the WPB strain onto Kentucky bluegrass in nitrogen-free medium. Compared to uninoculated control plants, the plants inoculated with WPB gained more dry weight (42%, p = 0.01) and more nitrogen content (37%, p = 0.04) in 50 days.

Original languageEnglish
Pages (from-to)669-674
Number of pages6
JournalBiology and Fertility of Soils
Volume45
Issue number6
DOIs
StatePublished - Jul 2009

Keywords

  • Auxin
  • Burkholderia vietnamiensis
  • Diazotrophic endophyte
  • Endophytes
  • IAA
  • Nitrogen fixation
  • Phylogenetic analysis
  • Plant growth promotion
  • Poplar trees
  • Populus
  • Turf grass

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