Abstract
An endophytic bacterium, wild poplar strain B (WPB), isolated from stems of wild cottonwood (Populus trichocarpa) was identified to Burkholderia vietnamiensis by analyzing the recA and rDNA genes. Phylogenetic analysis of the nifHDK cluster indicates that the WPB isolate shares high sequence similarity with known B. vietnamiensis strains. The nitrogenase activity of WPB was determined by a 15N2 incorporation assay and an acetylene reduction assay. WPB was also monitored for production of indole-3-acetic acid (IAA), a phytohormone which can promote plant growth, when incubated with L-tryptophan. In addition, its plant growth promotion capacity was assessed by inoculating the WPB strain onto Kentucky bluegrass in nitrogen-free medium. Compared to uninoculated control plants, the plants inoculated with WPB gained more dry weight (42%, p = 0.01) and more nitrogen content (37%, p = 0.04) in 50 days.
Original language | English |
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Pages (from-to) | 669-674 |
Number of pages | 6 |
Journal | Biology and Fertility of Soils |
Volume | 45 |
Issue number | 6 |
DOIs | |
State | Published - Jul 2009 |
Keywords
- Auxin
- Burkholderia vietnamiensis
- Diazotrophic endophyte
- Endophytes
- IAA
- Nitrogen fixation
- Phylogenetic analysis
- Plant growth promotion
- Poplar trees
- Populus
- Turf grass