TY - JOUR
T1 - A double antibody sandwich enzyme-linked immunosorbent assay for detection of secreted antigen 1 of Babesia microti using hamster model
AU - Luo, Yuzi
AU - Terkawi, Mohamad Alaa
AU - Jia, Honglin
AU - Aboge, Gabriel Oluga
AU - Goo, Youn Kyoung
AU - Cao, Shinuo
AU - Li, Yan
AU - Yu, Longzheng
AU - Ooka, Hideo
AU - Kamyingkird, Ketsarin
AU - Masatani, Tatsunori
AU - Zhang, Shoufa
AU - Nishikawa, Yoshifumi
AU - Igarashi, Ikuo
AU - Xuan, Xuenan
PY - 2012/2
Y1 - 2012/2
N2 - A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) targeting secreted antigen 1 of Babesia microti (BmSA1) was developed for detection of B. microti infection. The optimized DAS-ELISA was sensitive enough to detect circulating BmSA1 by day 2 post-infection, in sequential sera of a hamster infected with B. microti. This detection was 4. days earlier than antibody detection by indirect ELISA. The kinetics of circulating BmSA1 coincided with the profile of parasitemia. The specificity of this assay was evaluated using sera from animals experimentally infected with different species of Babesia. The DAS-ELISA had a higher sensitivity than the microscopic examination of Giemsa-stained blood smears for detection of the infection in hamsters. Taken together, these results indicated that BmSA1 could be a potential marker for surveillance of human babesiosis.
AB - A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) targeting secreted antigen 1 of Babesia microti (BmSA1) was developed for detection of B. microti infection. The optimized DAS-ELISA was sensitive enough to detect circulating BmSA1 by day 2 post-infection, in sequential sera of a hamster infected with B. microti. This detection was 4. days earlier than antibody detection by indirect ELISA. The kinetics of circulating BmSA1 coincided with the profile of parasitemia. The specificity of this assay was evaluated using sera from animals experimentally infected with different species of Babesia. The DAS-ELISA had a higher sensitivity than the microscopic examination of Giemsa-stained blood smears for detection of the infection in hamsters. Taken together, these results indicated that BmSA1 could be a potential marker for surveillance of human babesiosis.
KW - Babesia microti
KW - BmSA1
KW - Double antibody sandwich ELISA
KW - Secreted antigen
UR - http://www.scopus.com/inward/record.url?scp=84856031455&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2011.10.012
DO - 10.1016/j.exppara.2011.10.012
M3 - Article
C2 - 22085769
AN - SCOPUS:84856031455
SN - 0014-4894
VL - 130
SP - 178
EP - 182
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 2
ER -
