A facile and sensitive detection of pathogenic bacteria using magnetic nanoparticles and optical nanocrystal probes

Jinmyoung Joo; Changyong Yim; Donghoon Kwon; Jaejin Lee; Hwa Hui Shin; Hyung Joon Cha; Sangmin Jeon

doi:10.1039/c2an35369e

A facile and sensitive detection of pathogenic bacteria using magnetic nanoparticles and optical nanocrystal probes

Jinmyoung Joo, Changyong Yim, Donghoon Kwon, Jaejin Lee, Hwa Hui Shin, Hyung Joon Cha, Sangmin Jeon

Pohang University of Science and Technology

Research output: Contribution to journal › Article › peer-review

90 Scopus citations

Abstract

We report a facile and sensitive analytical method for the detection of pathogenic bacteria. Salmonella bacteria in milk were captured by antibody-conjugated magnetic nanoparticles (MNPs) and separated from analyte samples by applying an external magnetic field. The MNP-Salmonella complexes were re-dispersed in a buffer solution then exposed to antibody-immobilized TiO₂ nanocrystals (TNs), which absorb UV light. After magnetically separating the MNP-Salmonella-TN complexes from solution, the UV-Vis absorption spectrum of the unbound TN solution was obtained. Because the light absorption intensity was reversely proportional to the Salmonella concentration, the assay exhibited high sensitivity toward low concentrations of Salmonella bacteria. The detection limit of Salmonella in milk was found to be more than 100 cfu mL ^-1.

Original language	English
Pages (from-to)	3609-3612
Number of pages	4
Journal	The Analyst
Volume	137
Issue number	16
DOIs	https://doi.org/10.1039/c2an35369e
State	Published - 21 Aug 2012

Access to Document

10.1039/c2an35369e

Cite this

@article{0ad64538d18d498ab00985bfc6ed86b5,

title = "A facile and sensitive detection of pathogenic bacteria using magnetic nanoparticles and optical nanocrystal probes",

abstract = "We report a facile and sensitive analytical method for the detection of pathogenic bacteria. Salmonella bacteria in milk were captured by antibody-conjugated magnetic nanoparticles (MNPs) and separated from analyte samples by applying an external magnetic field. The MNP-Salmonella complexes were re-dispersed in a buffer solution then exposed to antibody-immobilized TiO2 nanocrystals (TNs), which absorb UV light. After magnetically separating the MNP-Salmonella-TN complexes from solution, the UV-Vis absorption spectrum of the unbound TN solution was obtained. Because the light absorption intensity was reversely proportional to the Salmonella concentration, the assay exhibited high sensitivity toward low concentrations of Salmonella bacteria. The detection limit of Salmonella in milk was found to be more than 100 cfu mL -1.",

author = "Jinmyoung Joo and Changyong Yim and Donghoon Kwon and Jaejin Lee and Shin, {Hwa Hui} and Cha, {Hyung Joon} and Sangmin Jeon",

year = "2012",

month = aug,

day = "21",

doi = "10.1039/c2an35369e",

language = "English",

volume = "137",

pages = "3609--3612",

journal = "The Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

number = "16",

}

TY - JOUR

T1 - A facile and sensitive detection of pathogenic bacteria using magnetic nanoparticles and optical nanocrystal probes

AU - Joo, Jinmyoung

AU - Yim, Changyong

AU - Kwon, Donghoon

AU - Lee, Jaejin

AU - Shin, Hwa Hui

AU - Cha, Hyung Joon

AU - Jeon, Sangmin

PY - 2012/8/21

Y1 - 2012/8/21

N2 - We report a facile and sensitive analytical method for the detection of pathogenic bacteria. Salmonella bacteria in milk were captured by antibody-conjugated magnetic nanoparticles (MNPs) and separated from analyte samples by applying an external magnetic field. The MNP-Salmonella complexes were re-dispersed in a buffer solution then exposed to antibody-immobilized TiO2 nanocrystals (TNs), which absorb UV light. After magnetically separating the MNP-Salmonella-TN complexes from solution, the UV-Vis absorption spectrum of the unbound TN solution was obtained. Because the light absorption intensity was reversely proportional to the Salmonella concentration, the assay exhibited high sensitivity toward low concentrations of Salmonella bacteria. The detection limit of Salmonella in milk was found to be more than 100 cfu mL -1.

AB - We report a facile and sensitive analytical method for the detection of pathogenic bacteria. Salmonella bacteria in milk were captured by antibody-conjugated magnetic nanoparticles (MNPs) and separated from analyte samples by applying an external magnetic field. The MNP-Salmonella complexes were re-dispersed in a buffer solution then exposed to antibody-immobilized TiO2 nanocrystals (TNs), which absorb UV light. After magnetically separating the MNP-Salmonella-TN complexes from solution, the UV-Vis absorption spectrum of the unbound TN solution was obtained. Because the light absorption intensity was reversely proportional to the Salmonella concentration, the assay exhibited high sensitivity toward low concentrations of Salmonella bacteria. The detection limit of Salmonella in milk was found to be more than 100 cfu mL -1.

UR - http://www.scopus.com/inward/record.url?scp=84863888202&partnerID=8YFLogxK

U2 - 10.1039/c2an35369e

DO - 10.1039/c2an35369e

M3 - Article

C2 - 22576145

AN - SCOPUS:84863888202

SN - 0003-2654

VL - 137

SP - 3609

EP - 3612

JO - The Analyst

JF - The Analyst

IS - 16

ER -

A facile and sensitive detection of pathogenic bacteria using magnetic nanoparticles and optical nanocrystal probes

Abstract

Access to Document

Other files and links

Fingerprint

Cite this