A highly discriminating quencher-free molecular beacon for probing DNA

Gil Tae Hwang, Young Jun Seo, Byeang Hyean Kim

Research output: Contribution to journalArticlepeer-review

179 Scopus citations

Abstract

We inserted a fluorene-labeled deoxyuridine derivative, synthesized using Sonogashira coupling, efficiently into the loop region of a DNA hairpin using phosphoramidite chemistry. This molecular beacon, which features no additional fluorescence quencher, discriminates between perfect and one-base-mismatched base pairing by changes in its fluorescence intensity. The discrimination factor is 14.7 for the recognition of a single (A/C) base mismatch.

Original languageEnglish
Pages (from-to)6528-6529
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number21
DOIs
StatePublished - 2 Jun 2004

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