A highly discriminating quencher-free molecular beacon for probing DNA

Gil Tae Hwang; Young Jun Seo; Byeang Hyean Kim

doi:10.1021/ja049795q

A highly discriminating quencher-free molecular beacon for probing DNA

Gil Tae Hwang, Young Jun Seo, Byeang Hyean Kim

Pohang University of Science and Technology

Research output: Contribution to journal › Article › peer-review

179 Scopus citations

Abstract

We inserted a fluorene-labeled deoxyuridine derivative, synthesized using Sonogashira coupling, efficiently into the loop region of a DNA hairpin using phosphoramidite chemistry. This molecular beacon, which features no additional fluorescence quencher, discriminates between perfect and one-base-mismatched base pairing by changes in its fluorescence intensity. The discrimination factor is 14.7 for the recognition of a single (A/C) base mismatch.

Original language	English
Pages (from-to)	6528-6529
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	126
Issue number	21
DOIs	https://doi.org/10.1021/ja049795q
State	Published - 2 Jun 2004

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title = "A highly discriminating quencher-free molecular beacon for probing DNA",

abstract = "We inserted a fluorene-labeled deoxyuridine derivative, synthesized using Sonogashira coupling, efficiently into the loop region of a DNA hairpin using phosphoramidite chemistry. This molecular beacon, which features no additional fluorescence quencher, discriminates between perfect and one-base-mismatched base pairing by changes in its fluorescence intensity. The discrimination factor is 14.7 for the recognition of a single (A/C) base mismatch.",

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AU - Hwang, Gil Tae

AU - Seo, Young Jun

AU - Kim, Byeang Hyean

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N2 - We inserted a fluorene-labeled deoxyuridine derivative, synthesized using Sonogashira coupling, efficiently into the loop region of a DNA hairpin using phosphoramidite chemistry. This molecular beacon, which features no additional fluorescence quencher, discriminates between perfect and one-base-mismatched base pairing by changes in its fluorescence intensity. The discrimination factor is 14.7 for the recognition of a single (A/C) base mismatch.

AB - We inserted a fluorene-labeled deoxyuridine derivative, synthesized using Sonogashira coupling, efficiently into the loop region of a DNA hairpin using phosphoramidite chemistry. This molecular beacon, which features no additional fluorescence quencher, discriminates between perfect and one-base-mismatched base pairing by changes in its fluorescence intensity. The discrimination factor is 14.7 for the recognition of a single (A/C) base mismatch.

UR - https://www.scopus.com/pages/publications/2542526068

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DO - 10.1021/ja049795q

M3 - Article

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SP - 6528

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 21

ER -

A highly discriminating quencher-free molecular beacon for probing DNA

Abstract

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