A new colorimetric method for determining the isomerization activity of sucrose isomerase

Sang Eun Park; Mee Hyun Cho; Jin Kyu Lim; Jong Sang Kim; Jeong Hwan Kim; Dae Young Kwon; Cheon Seok Park

doi:10.1271/bbb.60509

A new colorimetric method for determining the isomerization activity of sucrose isomerase

Sang Eun Park, Mee Hyun Cho, Jin Kyu Lim, Jong Sang Kim, Jeong Hwan Kim, Dae Young Kwon, Cheon Seok Park

School of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A new colorimetric method for determining the isomerization activity of sucrose isomerase was developed. This colorimetric method is based on the enzymatic reactions of invertase and glucose oxidase-peroxidase (GOD-POD). The main scheme for assaying sucrose isomerase activity is to degrade sucrose in the reaction mixture to glucose and fructose by invertase and to detect the concentration of glucose generated using GOD-POD. The concentrations of trehalulose and isomaltulose, reaction products of sucrose isomerase, are calculated from the concentration of glucose. This method allows rapid and accurate determination of the isomerization activity of sucrose isomerase without inhibition by hydrolysis activity.

Original language	English
Pages (from-to)	583-586
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	71
Issue number	2
DOIs	https://doi.org/10.1271/bbb.60509
State	Published - 2007

Keywords

Glucose oxidase-peroxidase
Invertase
Isomaltulose
Sucrose
Sucrose isomerase

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10.1271/bbb.60509

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abstract = "A new colorimetric method for determining the isomerization activity of sucrose isomerase was developed. This colorimetric method is based on the enzymatic reactions of invertase and glucose oxidase-peroxidase (GOD-POD). The main scheme for assaying sucrose isomerase activity is to degrade sucrose in the reaction mixture to glucose and fructose by invertase and to detect the concentration of glucose generated using GOD-POD. The concentrations of trehalulose and isomaltulose, reaction products of sucrose isomerase, are calculated from the concentration of glucose. This method allows rapid and accurate determination of the isomerization activity of sucrose isomerase without inhibition by hydrolysis activity.",

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AU - Park, Sang Eun

AU - Cho, Mee Hyun

AU - Lim, Jin Kyu

AU - Kim, Jong Sang

AU - Kim, Jeong Hwan

AU - Kwon, Dae Young

AU - Park, Cheon Seok

PY - 2007

Y1 - 2007

N2 - A new colorimetric method for determining the isomerization activity of sucrose isomerase was developed. This colorimetric method is based on the enzymatic reactions of invertase and glucose oxidase-peroxidase (GOD-POD). The main scheme for assaying sucrose isomerase activity is to degrade sucrose in the reaction mixture to glucose and fructose by invertase and to detect the concentration of glucose generated using GOD-POD. The concentrations of trehalulose and isomaltulose, reaction products of sucrose isomerase, are calculated from the concentration of glucose. This method allows rapid and accurate determination of the isomerization activity of sucrose isomerase without inhibition by hydrolysis activity.

AB - A new colorimetric method for determining the isomerization activity of sucrose isomerase was developed. This colorimetric method is based on the enzymatic reactions of invertase and glucose oxidase-peroxidase (GOD-POD). The main scheme for assaying sucrose isomerase activity is to degrade sucrose in the reaction mixture to glucose and fructose by invertase and to detect the concentration of glucose generated using GOD-POD. The concentrations of trehalulose and isomaltulose, reaction products of sucrose isomerase, are calculated from the concentration of glucose. This method allows rapid and accurate determination of the isomerization activity of sucrose isomerase without inhibition by hydrolysis activity.

KW - Glucose oxidase-peroxidase

KW - Invertase

KW - Isomaltulose

KW - Sucrose

KW - Sucrose isomerase

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

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ER -

A new colorimetric method for determining the isomerization activity of sucrose isomerase

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