A Novel Ultrasound Technique for Non-Invasive Assessment of Cell Differentiation

Wenbin Huang; Jinwook Kim; Kyngrim Kim; Saurabh Bakshi; John Williams; Pattie Matthieu; Elizabeth Loboa; Koping Kirk Shung; Qifa Zhou; Xiaoning Jiang

doi:10.1109/JSEN.2015.2477340

A Novel Ultrasound Technique for Non-Invasive Assessment of Cell Differentiation

Wenbin Huang, Jinwook Kim, Kyngrim Kim, Saurabh Bakshi, John Williams, Pattie Matthieu, Elizabeth Loboa, Koping Kirk Shung, Qifa Zhou, Xiaoning Jiang

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A novel technique for the characterization of mammalian cells during cell culture was studied using a lead magnesium niobate-lead titanate single crystal piezoelectric resonator. Tests were conducted to observe changes in material properties of human adipose derived stem cells during both proliferation and osteogenic differentiation. The resonator electrical impedance was recorded as a function of the cell acoustic impedance, an indicator of cell viscoelasticity. Observed electrical impedance change (in percentage) from day 1 to day 14 for human adipose derived stem cells undergoing chemical-induced osteogenic differentiation was ∼ 1.7× that observed for proliferating stem cells maintained in complete growth medium.

Original language	English
Article number	7270277
Pages (from-to)	61-68
Number of pages	8
Journal	IEEE Sensors Journal
Volume	16
Issue number	1
DOIs	https://doi.org/10.1109/JSEN.2015.2477340
State	Published - 1 Jan 2016

Keywords

PMN-PT piezoelectric resonator
electrical impedance measurement
in-situ cell differentiation monitoring
osteogenic differentiation

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10.1109/JSEN.2015.2477340

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abstract = "A novel technique for the characterization of mammalian cells during cell culture was studied using a lead magnesium niobate-lead titanate single crystal piezoelectric resonator. Tests were conducted to observe changes in material properties of human adipose derived stem cells during both proliferation and osteogenic differentiation. The resonator electrical impedance was recorded as a function of the cell acoustic impedance, an indicator of cell viscoelasticity. Observed electrical impedance change (in percentage) from day 1 to day 14 for human adipose derived stem cells undergoing chemical-induced osteogenic differentiation was ∼ 1.7× that observed for proliferating stem cells maintained in complete growth medium.",

keywords = "PMN-PT piezoelectric resonator, electrical impedance measurement, in-situ cell differentiation monitoring, osteogenic differentiation",

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AU - Kim, Jinwook

AU - Kim, Kyngrim

AU - Bakshi, Saurabh

AU - Williams, John

AU - Matthieu, Pattie

AU - Loboa, Elizabeth

AU - Shung, Koping Kirk

AU - Zhou, Qifa

AU - Jiang, Xiaoning

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AB - A novel technique for the characterization of mammalian cells during cell culture was studied using a lead magnesium niobate-lead titanate single crystal piezoelectric resonator. Tests were conducted to observe changes in material properties of human adipose derived stem cells during both proliferation and osteogenic differentiation. The resonator electrical impedance was recorded as a function of the cell acoustic impedance, an indicator of cell viscoelasticity. Observed electrical impedance change (in percentage) from day 1 to day 14 for human adipose derived stem cells undergoing chemical-induced osteogenic differentiation was ∼ 1.7× that observed for proliferating stem cells maintained in complete growth medium.

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A Novel Ultrasound Technique for Non-Invasive Assessment of Cell Differentiation

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Keywords

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