A RAPD-PCR Method for the Rapid Detection of Bacillus Cereus

Jiyeon Lee; Gun Hee Kwon; Jae Yong Park; Cheon Seok Park; Dae Young Kwon; Jinkyu Lim; Jong Sang Kim; Jeong Hwan Kim

doi:10.4014/jmb.1008.08031

A RAPD-PCR Method for the Rapid Detection of Bacillus Cereus

Jiyeon Lee, Gun Hee Kwon, Jae Yong Park, Cheon Seok Park, Dae Young Kwon, Jinkyu Lim, Jong Sang Kim, Jeong Hwan Kim

School of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 10²/g sample.

Original language	English
Pages (from-to)	274-276
Number of pages	3
Journal	Journal of Microbiology and Biotechnology
Volume	21
Issue number	3
DOIs	https://doi.org/10.4014/jmb.1008.08031
State	Published - Mar 2011

Keywords

Bacillus cereus
Food poisoning
Rapd-pcr
Rapid detection

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10.4014/jmb.1008.08031

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abstract = "Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 102/g sample.",

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AU - Lee, Jiyeon

AU - Kwon, Gun Hee

AU - Park, Jae Yong

AU - Park, Cheon Seok

AU - Kwon, Dae Young

AU - Lim, Jinkyu

AU - Kim, Jong Sang

AU - Kim, Jeong Hwan

PY - 2011/3

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AB - Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 102/g sample.

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A RAPD-PCR Method for the Rapid Detection of Bacillus Cereus

Abstract

Keywords

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