A simple gel electrophoresis method for separating polyhedral gold nanoparticles

Suhee Kim, Hye Jin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this paper, a simple approach to separate differently shaped and sized polyhedral gold nanoparticles (NPs) within colloidal solutions via gel electrophoresis is described. Gel running parameters for separating efficiently gold NPs including gel composition, added surfactant types and applied voltage were investigated. The plasmonic properties and physical structure of the separated NPs extracted from the gel matrix were then investigated using transmission electron microscopy (TEM) and UV-vis spectrophotometry respectively. Data analysis revealed that gel electrophoresis conditions of a 1.5 % agarose gel with 0.1 % sodium dodecyl sulfate (SDS) surfactant under an applied voltage of 100 V resulted in the selective isolation of ∼ 50 nm polyhedral shaped gold nanoparticles. Further efforts are underway to apply the method to purify biomolecule-conjugated polyhedral Au NPs that can be readily used for NP-enhanced biosensing platforms.

Original languageEnglish
Title of host publicationInternational Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015
EditorsDonghyun Kim, Min-Gon Kim, Seung-Han Park
PublisherSPIE
ISBN (Electronic)9781628416794
DOIs
StatePublished - 2015
Event2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015 - Jeju, Korea, Republic of
Duration: 25 Feb 201527 Feb 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9523
ISSN (Print)1605-7422

Conference

Conference2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015
Country/TerritoryKorea, Republic of
CityJeju
Period25/02/1527/02/15

Keywords

  • Agarose
  • Gel electrophoresis
  • Nanobiosensing materials
  • Polyhedral gold nanoparticles
  • Separation
  • Surfactants

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