A strategy to enhance the binding affinity of fluorophore–aptamer pairs for RNA tagging with neomycin conjugation

Jongho Jeon; Kyung Hyun Lee; Jianghong Rao

doi:10.1039/c2cc34498j

A strategy to enhance the binding affinity of fluorophore–aptamer pairs for RNA tagging with neomycin conjugation

Jongho Jeon, Kyung Hyun Lee, Jianghong Rao

Department of Applied Chemistry

Stanford University

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Fluorogenic sulforhodamine–neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250–400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.

Original language	English
Pages (from-to)	10034-10036
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	80
DOIs	https://doi.org/10.1039/c2cc34498j
State	Published - 12 Sep 2012

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title = "A strategy to enhance the binding affinity of fluorophore–aptamer pairs for RNA tagging with neomycin conjugation",

abstract = "Fluorogenic sulforhodamine–neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250–400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.",

author = "Jongho Jeon and Lee, {Kyung Hyun} and Jianghong Rao",

year = "2012",

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AU - Jeon, Jongho

AU - Lee, Kyung Hyun

AU - Rao, Jianghong

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Y1 - 2012/9/12

N2 - Fluorogenic sulforhodamine–neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250–400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.

AB - Fluorogenic sulforhodamine–neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250–400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.

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DO - 10.1039/c2cc34498j

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JO - Chemical Communications

JF - Chemical Communications

IS - 80

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A strategy to enhance the binding affinity of fluorophore–aptamer pairs for RNA tagging with neomycin conjugation

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