A strategy to enhance the binding affinity of fluorophore–aptamer pairs for RNA tagging with neomycin conjugation

Jongho Jeon; Kyung Hyun Lee; Jianghong Rao

doi:10.1039/c2cc34498j

A strategy to enhance the binding affinity of fluorophore–aptamer pairs for RNA tagging with neomycin conjugation

Jongho Jeon
, Kyung Hyun Lee
, Jianghong Rao

Department of Applied Chemistry

Stanford University

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Fluorogenic sulforhodamine–neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250–400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.

Original language	English
Pages (from-to)	10034-10036
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	80
DOIs	https://doi.org/10.1039/c2cc34498j
State	Published - 12 Sep 2012

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title = "A strategy to enhance the binding affinity of fluorophore–aptamer pairs for RNA tagging with neomycin conjugation",

abstract = "Fluorogenic sulforhodamine–neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250–400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.",

author = "Jongho Jeon and Lee, \{Kyung Hyun\} and Jianghong Rao",

year = "2012",

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doi = "10.1039/c2cc34498j",

language = "English",

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T1 - A strategy to enhance the binding affinity of fluorophore–aptamer pairs for RNA tagging with neomycin conjugation

AU - Jeon, Jongho

AU - Lee, Kyung Hyun

AU - Rao, Jianghong

PY - 2012/9/12

Y1 - 2012/9/12

N2 - Fluorogenic sulforhodamine–neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250–400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.

AB - Fluorogenic sulforhodamine–neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250–400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.

UR - https://www.scopus.com/pages/publications/84866355636

U2 - 10.1039/c2cc34498j

DO - 10.1039/c2cc34498j

M3 - Article

C2 - 22951899

AN - SCOPUS:84866355636

SN - 1359-7345

VL - 48

SP - 10034

EP - 10036

JO - Chemical Communications

JF - Chemical Communications

IS - 80

ER -

A strategy to enhance the binding affinity of fluorophore–aptamer pairs for RNA tagging with neomycin conjugation

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