A tissue embedding technique for measuring the structure of hairy root mats of Tagetes erecta

Won Sik Shin; M. A. Hjortsø

doi:10.1007/BF02707067

A tissue embedding technique for measuring the structure of hairy root mats of Tagetes erecta

Won Sik Shin, M. A. Hjortsø

Louisiana State University

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A tissue embedding protocol which preserves root diameter was developed for hairy root mats of Tagetes erecta. Embedded root mats were sectioned, stained and the cut surfaces were subjected to image analysis to determine the void fraction of the root mats. Void fractions of mats of different ages, grown from inocula containing different numbers of tips, were analyzed. The method was found to give reproducible values for the void fractions, exhibiting reasonable trends with respect to initial tip number and mat age.

Original language	English
Pages (from-to)	150-156
Number of pages	7
Journal	Korean Journal of Chemical Engineering
Volume	15
Issue number	2
DOIs	https://doi.org/10.1007/BF02707067
State	Published - Mar 1998

Keywords

Embedding
Hairy Root Mat
Image Analysis
Plant Tissue Culture
Tagetes erecta

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10.1007/BF02707067

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title = "A tissue embedding technique for measuring the structure of hairy root mats of Tagetes erecta",

abstract = "A tissue embedding protocol which preserves root diameter was developed for hairy root mats of Tagetes erecta. Embedded root mats were sectioned, stained and the cut surfaces were subjected to image analysis to determine the void fraction of the root mats. Void fractions of mats of different ages, grown from inocula containing different numbers of tips, were analyzed. The method was found to give reproducible values for the void fractions, exhibiting reasonable trends with respect to initial tip number and mat age.",

keywords = "Embedding, Hairy Root Mat, Image Analysis, Plant Tissue Culture, Tagetes erecta",

author = "Shin, {Won Sik} and Hjorts{\o}, {M. A.}",

year = "1998",

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AU - Hjortsø, M. A.

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N2 - A tissue embedding protocol which preserves root diameter was developed for hairy root mats of Tagetes erecta. Embedded root mats were sectioned, stained and the cut surfaces were subjected to image analysis to determine the void fraction of the root mats. Void fractions of mats of different ages, grown from inocula containing different numbers of tips, were analyzed. The method was found to give reproducible values for the void fractions, exhibiting reasonable trends with respect to initial tip number and mat age.

AB - A tissue embedding protocol which preserves root diameter was developed for hairy root mats of Tagetes erecta. Embedded root mats were sectioned, stained and the cut surfaces were subjected to image analysis to determine the void fraction of the root mats. Void fractions of mats of different ages, grown from inocula containing different numbers of tips, were analyzed. The method was found to give reproducible values for the void fractions, exhibiting reasonable trends with respect to initial tip number and mat age.

KW - Embedding

KW - Hairy Root Mat

KW - Image Analysis

KW - Plant Tissue Culture

KW - Tagetes erecta

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ER -

A tissue embedding technique for measuring the structure of hairy root mats of Tagetes erecta

Abstract

Keywords

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