A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission

Nam Kyung Yu; Hyoung F. Kim; Jaehoon Shim; Somi Kim; Dae Won Kim; Chuljung Kwak; Su Eon Sim; Jun Hyeok Choi; Seohee Ahn; Juyoun Yoo; Sun Lim Choi; Deok Jin Jang; Chae Seok Lim; Yong Seok Lee; Chulhun Kang; Soo Young Choi; Bong Kiun Kaang

doi:10.1038/srep22892

A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission

Nam Kyung Yu, Hyoung F. Kim, Jaehoon Shim, Somi Kim, Dae Won Kim, Chuljung Kwak, Su Eon Sim, Jun Hyeok Choi, Seohee Ahn, Juyoun Yoo, Sun Lim Choi, Deok Jin Jang, Chae Seok Lim, Yong Seok Lee, Chulhun Kang, Soo Young Choi, Bong Kiun Kaang

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Cell-permeable proteins are emerging as unconventional regulators of signal transduction and providing a potential for therapeutic applications. However, only a few of them are identified and studied in detail. We identify a novel cell-permeable protein, mouse LLP homolog (mLLP), and uncover its roles in regulating neural development. We found that mLLP is strongly expressed in developing nervous system and that mLLP knockdown or overexpression during maturation of cultured neurons affected the neuronal growth and synaptic transmission. Interestingly, extracellular addition of mLLP protein enhanced dendritic arborization, demonstrating the non-cell-autonomous effect of mLLP. Moreover, mLLP interacts with CCCTC-binding factor (CTCF) as well as transcriptional machineries and modulates gene expression involved in neuronal growth. Together, these results illustrate the characteristics and roles of previously unknown cell-permeable protein mLLP in modulating neural development.

Original language	English
Article number	22892
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep22892
State	Published - 10 Mar 2016

Access to Document

10.1038/srep22892

Cite this

Yu, N. K., Kim, H. F., Shim, J., Kim, S., Kim, D. W., Kwak, C., Sim, S. E., Choi, J. H., Ahn, S., Yoo, J., Choi, S. L., Jang, D. J., Lim, C. S., Lee, Y. S., Kang, C., Choi, S. Y., & Kaang, B. K. (2016). A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission. Scientific Reports, 6, Article 22892. https://doi.org/10.1038/srep22892

@article{c410866dffc446f38405d4975ad4a2f8,

title = "A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission",

abstract = "Cell-permeable proteins are emerging as unconventional regulators of signal transduction and providing a potential for therapeutic applications. However, only a few of them are identified and studied in detail. We identify a novel cell-permeable protein, mouse LLP homolog (mLLP), and uncover its roles in regulating neural development. We found that mLLP is strongly expressed in developing nervous system and that mLLP knockdown or overexpression during maturation of cultured neurons affected the neuronal growth and synaptic transmission. Interestingly, extracellular addition of mLLP protein enhanced dendritic arborization, demonstrating the non-cell-autonomous effect of mLLP. Moreover, mLLP interacts with CCCTC-binding factor (CTCF) as well as transcriptional machineries and modulates gene expression involved in neuronal growth. Together, these results illustrate the characteristics and roles of previously unknown cell-permeable protein mLLP in modulating neural development.",

author = "Yu, {Nam Kyung} and Kim, {Hyoung F.} and Jaehoon Shim and Somi Kim and Kim, {Dae Won} and Chuljung Kwak and Sim, {Su Eon} and Choi, {Jun Hyeok} and Seohee Ahn and Juyoun Yoo and Choi, {Sun Lim} and Jang, {Deok Jin} and Lim, {Chae Seok} and Lee, {Yong Seok} and Chulhun Kang and Choi, {Soo Young} and Kaang, {Bong Kiun}",

year = "2016",

month = mar,

day = "10",

doi = "10.1038/srep22892",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission

AU - Yu, Nam Kyung

AU - Kim, Hyoung F.

AU - Shim, Jaehoon

AU - Kim, Somi

AU - Kim, Dae Won

AU - Kwak, Chuljung

AU - Sim, Su Eon

AU - Choi, Jun Hyeok

AU - Ahn, Seohee

AU - Yoo, Juyoun

AU - Choi, Sun Lim

AU - Jang, Deok Jin

AU - Lim, Chae Seok

AU - Lee, Yong Seok

AU - Kang, Chulhun

AU - Choi, Soo Young

AU - Kaang, Bong Kiun

PY - 2016/3/10

Y1 - 2016/3/10

N2 - Cell-permeable proteins are emerging as unconventional regulators of signal transduction and providing a potential for therapeutic applications. However, only a few of them are identified and studied in detail. We identify a novel cell-permeable protein, mouse LLP homolog (mLLP), and uncover its roles in regulating neural development. We found that mLLP is strongly expressed in developing nervous system and that mLLP knockdown or overexpression during maturation of cultured neurons affected the neuronal growth and synaptic transmission. Interestingly, extracellular addition of mLLP protein enhanced dendritic arborization, demonstrating the non-cell-autonomous effect of mLLP. Moreover, mLLP interacts with CCCTC-binding factor (CTCF) as well as transcriptional machineries and modulates gene expression involved in neuronal growth. Together, these results illustrate the characteristics and roles of previously unknown cell-permeable protein mLLP in modulating neural development.

AB - Cell-permeable proteins are emerging as unconventional regulators of signal transduction and providing a potential for therapeutic applications. However, only a few of them are identified and studied in detail. We identify a novel cell-permeable protein, mouse LLP homolog (mLLP), and uncover its roles in regulating neural development. We found that mLLP is strongly expressed in developing nervous system and that mLLP knockdown or overexpression during maturation of cultured neurons affected the neuronal growth and synaptic transmission. Interestingly, extracellular addition of mLLP protein enhanced dendritic arborization, demonstrating the non-cell-autonomous effect of mLLP. Moreover, mLLP interacts with CCCTC-binding factor (CTCF) as well as transcriptional machineries and modulates gene expression involved in neuronal growth. Together, these results illustrate the characteristics and roles of previously unknown cell-permeable protein mLLP in modulating neural development.

UR - http://www.scopus.com/inward/record.url?scp=84960884775&partnerID=8YFLogxK

U2 - 10.1038/srep22892

DO - 10.1038/srep22892

M3 - Article

C2 - 26961175

AN - SCOPUS:84960884775

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 22892

ER -

A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission

Abstract

Access to Document

Other files and links

Fingerprint

Cite this