Ac/Ds-mediated gene trap systems for functional genomics in rice

B. I. Je; C. M. Kim; Su Hyun Park; Sung Han Park; Y. J. Na; J. J. Lee; B. G. Oh; N. M. Hee; G. H. Yi; H. Y. Kim; C. D. Han

doi:10.1142/9789812814319_0137

Ac/Ds-mediated gene trap systems for functional genomics in rice

B. I. Je, C. M. Kim, Su Hyun Park, Sung Han Park, Y. J. Na, J. J. Lee, B. G. Oh, N. M. Hee, G. H. Yi, H. Y. Kim, C. D. Han

Department of Farm Management

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

We have established Ac/Ds-mediated gene trap systems in rice. The efficiency of these gene-tagging systems has been tested by producing transgenic rice plants carrying a simple and single T-DNA insert. High Ds mobility and effective gene detection implied that an Ac/Ds family would be an excellent genetic tool for use in functional genomics in rice. To produce a large population of Ds transposants, single-copy Ds and Ac “starter” lines were produced. Each Ds starter line was examined for transposition efficiency by crossing it with an Ac line. A couple of Ds lines (called Super J) showed that more than 50% of the F₂ progenies demonstrated germinal transmission of transposed Ds elements.

Original language	English
Title of host publication	Advances In Rice Genetics (In 2 Parts)
Publisher	World Scientific Publishing Co.
Pages	365-366
Number of pages	2
ISBN (Electronic)	9789812814319
DOIs	https://doi.org/10.1142/9789812814319_0137
State	Published - 1 Jan 2008

Access to Document

10.1142/9789812814319_0137

Cite this

@inbook{cd3a74b5f22949a095a434105d8fddb7,

title = "Ac/Ds-mediated gene trap systems for functional genomics in rice",

abstract = "We have established Ac/Ds-mediated gene trap systems in rice. The efficiency of these gene-tagging systems has been tested by producing transgenic rice plants carrying a simple and single T-DNA insert. High Ds mobility and effective gene detection implied that an Ac/Ds family would be an excellent genetic tool for use in functional genomics in rice. To produce a large population of Ds transposants, single-copy Ds and Ac “starter” lines were produced. Each Ds starter line was examined for transposition efficiency by crossing it with an Ac line. A couple of Ds lines (called Super J) showed that more than 50% of the F2 progenies demonstrated germinal transmission of transposed Ds elements.",

author = "Je, {B. I.} and Kim, {C. M.} and Park, {Su Hyun} and Park, {Sung Han} and Na, {Y. J.} and Lee, {J. J.} and Oh, {B. G.} and Hee, {N. M.} and Yi, {G. H.} and Kim, {H. Y.} and Han, {C. D.}",

note = "Publisher Copyright: {\textcopyright} International Rice Research Institute 2003.",

year = "2008",

month = jan,

day = "1",

doi = "10.1142/9789812814319_0137",

language = "English",

pages = "365--366",

booktitle = "Advances In Rice Genetics (In 2 Parts)",

publisher = "World Scientific Publishing Co.",

}

TY - CHAP

T1 - Ac/Ds-mediated gene trap systems for functional genomics in rice

AU - Je, B. I.

AU - Kim, C. M.

AU - Park, Su Hyun

AU - Park, Sung Han

AU - Na, Y. J.

AU - Lee, J. J.

AU - Oh, B. G.

AU - Hee, N. M.

AU - Yi, G. H.

AU - Kim, H. Y.

AU - Han, C. D.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - We have established Ac/Ds-mediated gene trap systems in rice. The efficiency of these gene-tagging systems has been tested by producing transgenic rice plants carrying a simple and single T-DNA insert. High Ds mobility and effective gene detection implied that an Ac/Ds family would be an excellent genetic tool for use in functional genomics in rice. To produce a large population of Ds transposants, single-copy Ds and Ac “starter” lines were produced. Each Ds starter line was examined for transposition efficiency by crossing it with an Ac line. A couple of Ds lines (called Super J) showed that more than 50% of the F2 progenies demonstrated germinal transmission of transposed Ds elements.

AB - We have established Ac/Ds-mediated gene trap systems in rice. The efficiency of these gene-tagging systems has been tested by producing transgenic rice plants carrying a simple and single T-DNA insert. High Ds mobility and effective gene detection implied that an Ac/Ds family would be an excellent genetic tool for use in functional genomics in rice. To produce a large population of Ds transposants, single-copy Ds and Ac “starter” lines were produced. Each Ds starter line was examined for transposition efficiency by crossing it with an Ac line. A couple of Ds lines (called Super J) showed that more than 50% of the F2 progenies demonstrated germinal transmission of transposed Ds elements.

UR - http://www.scopus.com/inward/record.url?scp=85153662200&partnerID=8YFLogxK

U2 - 10.1142/9789812814319_0137

DO - 10.1142/9789812814319_0137

M3 - Chapter

AN - SCOPUS:85153662200

SP - 365

EP - 366

BT - Advances In Rice Genetics (In 2 Parts)

PB - World Scientific Publishing Co.

ER -

Ac/Ds-mediated gene trap systems for functional genomics in rice

Abstract

Access to Document

Other files and links

Fingerprint

Cite this