TY - JOUR
T1 - Advances and Challenges of Sensing in Water Using CRISPR-Cas Technology
AU - Safarkhani, Moein
AU - Farasati Far, Bahareh
AU - Kim, Su Hyeon
AU - Makvandi, Pooyan
AU - Park, Mi Kyung
AU - Huh, Yun Suk
AU - Rabiee, Navid
N1 - Publisher Copyright:
© 2024 American Chemical Society.
PY - 2023
Y1 - 2023
N2 - The groundbreaking gene-editing mechanism, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), paired with the protein Cas9, has significantly advanced the realms of biology, medicine, and agriculture. Through its precision in modifying genetic sequences, CRISPR holds the potential to alter the trajectory of genetic disorders and accelerate advancements in agriculture. While its therapeutic potential is profound, the technology also invites ethical debates centered on responsible use and equity in access. Parallelly, in the environmental monitoring sphere and sensing in water, especially biosensors have been instrumental in evaluating natural water sources’ quality. These biosensors, integrating biological components with detection techniques, have the potential to revolutionize healthcare by providing rapid and minimally invasive diagnostic methods. However, the design and application of these sensors bring forth challenges, especially in ensuring sensitivity, selectivity, and ethical data handling. This article delves into the prospective use of CRISPR-Cas technology for sensing in water, exploring its capabilities in detecting diverse biomarkers, hazardous substances, and varied reactions in water and wastewater systems.
AB - The groundbreaking gene-editing mechanism, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), paired with the protein Cas9, has significantly advanced the realms of biology, medicine, and agriculture. Through its precision in modifying genetic sequences, CRISPR holds the potential to alter the trajectory of genetic disorders and accelerate advancements in agriculture. While its therapeutic potential is profound, the technology also invites ethical debates centered on responsible use and equity in access. Parallelly, in the environmental monitoring sphere and sensing in water, especially biosensors have been instrumental in evaluating natural water sources’ quality. These biosensors, integrating biological components with detection techniques, have the potential to revolutionize healthcare by providing rapid and minimally invasive diagnostic methods. However, the design and application of these sensors bring forth challenges, especially in ensuring sensitivity, selectivity, and ethical data handling. This article delves into the prospective use of CRISPR-Cas technology for sensing in water, exploring its capabilities in detecting diverse biomarkers, hazardous substances, and varied reactions in water and wastewater systems.
KW - CRISPR-Cas
KW - biosensor
KW - environmental science
KW - point-of-care
KW - sensor
UR - http://www.scopus.com/inward/record.url?scp=85189501692&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.3c01689
DO - 10.1021/acsbiomaterials.3c01689
M3 - Review article
AN - SCOPUS:85189501692
SN - 2373-9878
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
ER -