Agonistic activities to the benzodiazepine receptor by extracts of medicinal plants(II). Activities of component and active fractions from Gastrodia eleta

J. H. Ha; D. U. Lee; C. S. Yong; J. A. Kim; K. Huh

Agonistic activities to the benzodiazepine receptor by extracts of medicinal plants(II). Activities of component and active fractions from Gastrodia eleta

J. H. Ha, D. U. Lee, C. S. Yong, J. A. Kim, K. Huh

Department of Medicine

Dongguk University

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In order to find active ingradients having an agonistic activity to benzodiazepine receptor from Gastrodia elata Blume (Orchidaceae) which has been used as an anticonvulsant in oriental medicine, one component and some fractions were separated from the butanol extract of the rhizomes of this plant and evaluated for their activities on GABA/benzodiazepine receptor in vitro. As a result, one crude mixture (F4f) obtained from the most active fraction (F4) inhibited significantly the binding of [³H]Ro15-1788, a selective benzodiazepine receptor antagonist, to benzodiazepine receptor of rat cortices. GABA significantly enhanced the inhibition of [³H]flunitrazepam binding by F4f, and this positive GABA shift supported the strong possibility of the agonistic activity of F4f to benzodiazepine receptor.

Original language	English
Pages (from-to)	284-289
Number of pages	6
Journal	Korean Journal of Pharmacognosy
Volume	30
Issue number	3
State	Published - 1999

Keywords

Anticonvulsant
Benzodiazepine
GABA
Gatrodia elata Blume
Receptor agonist

Cite this

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title = "Agonistic activities to the benzodiazepine receptor by extracts of medicinal plants(II). Activities of component and active fractions from Gastrodia eleta",

abstract = "In order to find active ingradients having an agonistic activity to benzodiazepine receptor from Gastrodia elata Blume (Orchidaceae) which has been used as an anticonvulsant in oriental medicine, one component and some fractions were separated from the butanol extract of the rhizomes of this plant and evaluated for their activities on GABA/benzodiazepine receptor in vitro. As a result, one crude mixture (F4f) obtained from the most active fraction (F4) inhibited significantly the binding of [3H]Ro15-1788, a selective benzodiazepine receptor antagonist, to benzodiazepine receptor of rat cortices. GABA significantly enhanced the inhibition of [3H]flunitrazepam binding by F4f, and this positive GABA shift supported the strong possibility of the agonistic activity of F4f to benzodiazepine receptor.",

keywords = "Anticonvulsant, Benzodiazepine, GABA, Gatrodia elata Blume, Receptor agonist",

author = "Ha, {J. H.} and Lee, {D. U.} and Yong, {C. S.} and Kim, {J. A.} and K. Huh",

year = "1999",

language = "English",

volume = "30",

pages = "284--289",

journal = "Korean Journal of Pharmacognosy",

issn = "0253-3073",

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TY - JOUR

T1 - Agonistic activities to the benzodiazepine receptor by extracts of medicinal plants(II). Activities of component and active fractions from Gastrodia eleta

AU - Ha, J. H.

AU - Lee, D. U.

AU - Yong, C. S.

AU - Kim, J. A.

AU - Huh, K.

PY - 1999

Y1 - 1999

N2 - In order to find active ingradients having an agonistic activity to benzodiazepine receptor from Gastrodia elata Blume (Orchidaceae) which has been used as an anticonvulsant in oriental medicine, one component and some fractions were separated from the butanol extract of the rhizomes of this plant and evaluated for their activities on GABA/benzodiazepine receptor in vitro. As a result, one crude mixture (F4f) obtained from the most active fraction (F4) inhibited significantly the binding of [3H]Ro15-1788, a selective benzodiazepine receptor antagonist, to benzodiazepine receptor of rat cortices. GABA significantly enhanced the inhibition of [3H]flunitrazepam binding by F4f, and this positive GABA shift supported the strong possibility of the agonistic activity of F4f to benzodiazepine receptor.

AB - In order to find active ingradients having an agonistic activity to benzodiazepine receptor from Gastrodia elata Blume (Orchidaceae) which has been used as an anticonvulsant in oriental medicine, one component and some fractions were separated from the butanol extract of the rhizomes of this plant and evaluated for their activities on GABA/benzodiazepine receptor in vitro. As a result, one crude mixture (F4f) obtained from the most active fraction (F4) inhibited significantly the binding of [3H]Ro15-1788, a selective benzodiazepine receptor antagonist, to benzodiazepine receptor of rat cortices. GABA significantly enhanced the inhibition of [3H]flunitrazepam binding by F4f, and this positive GABA shift supported the strong possibility of the agonistic activity of F4f to benzodiazepine receptor.

KW - Anticonvulsant

KW - Benzodiazepine

KW - GABA

KW - Gatrodia elata Blume

KW - Receptor agonist

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M3 - Article

AN - SCOPUS:0032713916

SN - 0253-3073

VL - 30

SP - 284

EP - 289

JO - Korean Journal of Pharmacognosy

JF - Korean Journal of Pharmacognosy

IS - 3

ER -

Agonistic activities to the benzodiazepine receptor by extracts of medicinal plants(II). Activities of component and active fractions from Gastrodia eleta

Abstract

Keywords

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