Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene

C. K. Kim; J. D. Chung; S. H. Park; A. M. Burrell; K. K. Kamo; D. H. Byrne

doi:10.1023/B:TICU.0000022529.16697.90

Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene

C. K. Kim, J. D. Chung, S. H. Park, A. M. Burrell, K. K. Kamo, D. H. Byrne

Department of Horticultural Science

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Embryogenic calluses of Rosa hybrida cultivar Tineke were transformed with Agrobacterium tumefaciens strain LBA4404 containing the binary vector pBIN m-gfp5-ER into which the virE/virG genes had been inserted. Visualization of GFP-expressing cells enabled visual selection of dividing, embryogenic cell clusters that were transgenic. When the Agrobacterium strain with the bifunctional fusion marker containing additional virE/virG genes was used, the number of green fluorescent calluses increased. Transformation of the GFP-expressing rose plants was confirmed by Southern blot analysis.

Original language	English
Pages (from-to)	107-111
Number of pages	5
Journal	Plant Cell, Tissue and Organ Culture
Volume	78
Issue number	2
DOIs	https://doi.org/10.1023/B:TICU.0000022529.16697.90
State	Published - Aug 2004

Keywords

Genetic engineering
Marker gene
Rose
Transgenic plants
vir gene

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10.1023/B:TICU.0000022529.16697.90

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title = "Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene",

abstract = "Embryogenic calluses of Rosa hybrida cultivar Tineke were transformed with Agrobacterium tumefaciens strain LBA4404 containing the binary vector pBIN m-gfp5-ER into which the virE/virG genes had been inserted. Visualization of GFP-expressing cells enabled visual selection of dividing, embryogenic cell clusters that were transgenic. When the Agrobacterium strain with the bifunctional fusion marker containing additional virE/virG genes was used, the number of green fluorescent calluses increased. Transformation of the GFP-expressing rose plants was confirmed by Southern blot analysis.",

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year = "2004",

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AU - Kim, C. K.

AU - Chung, J. D.

AU - Park, S. H.

AU - Burrell, A. M.

AU - Kamo, K. K.

AU - Byrne, D. H.

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AB - Embryogenic calluses of Rosa hybrida cultivar Tineke were transformed with Agrobacterium tumefaciens strain LBA4404 containing the binary vector pBIN m-gfp5-ER into which the virE/virG genes had been inserted. Visualization of GFP-expressing cells enabled visual selection of dividing, embryogenic cell clusters that were transgenic. When the Agrobacterium strain with the bifunctional fusion marker containing additional virE/virG genes was used, the number of green fluorescent calluses increased. Transformation of the GFP-expressing rose plants was confirmed by Southern blot analysis.

KW - Genetic engineering

KW - Marker gene

KW - Rose

KW - Transgenic plants

KW - vir gene

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Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene

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Keywords

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