TY - JOUR
T1 - Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple Orchards
AU - Lee, Ju Heon
AU - Kim, Youngsoo
AU - Park, Jong Taek
AU - Lee, Dong Hyuk
AU - Jung, Hee Young
N1 - Publisher Copyright:
© 2024 THE KOREAN SOCIETY OF MYCOLOGY.
PY - 2024/12
Y1 - 2024/12
N2 - A fungus was isolated from ambrosia beetles collected using beetle traps in an apple orchard in Gunwi-gun, Daegu, South Korea. This fungal strain was termed ARI-24-A5, and was identified through morphological characterization and molecular phylogenetic analysis. After 8 d of incubation on potato dextrose agar (PDA), ARI-24-A5 exhibited gray-to-olive coloration, abundant aerial mycelia, and a colony diameter of 72.0–79.0 mm. Morphologically, the aleurioconidiophores formed monilioid chain structures, and the size of the aleurioconidia was 11.1 × 10.8 μm. For precise identification, molecular phylogenetic analysis was performed using the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF1-α), small subunit of nuclear ribosomal RNA (SSU), and RNA polymerase II subunit 1 (RPB1) gene sequences. The overall analysis confirmed that ARI-24-A5 belongs to the genus Ambrosiella, which is known for its symbiotic relationship with ambrosia beetles. In the phylogenetic tree, ARI-24-A5 shared the same taxonomic position as A. catenulata and its morphological characteristics were consistent with those of this species. Therefore, ARI-24-A5 was identified as A. catenulata, making this the first record of this species in South Korea.
AB - A fungus was isolated from ambrosia beetles collected using beetle traps in an apple orchard in Gunwi-gun, Daegu, South Korea. This fungal strain was termed ARI-24-A5, and was identified through morphological characterization and molecular phylogenetic analysis. After 8 d of incubation on potato dextrose agar (PDA), ARI-24-A5 exhibited gray-to-olive coloration, abundant aerial mycelia, and a colony diameter of 72.0–79.0 mm. Morphologically, the aleurioconidiophores formed monilioid chain structures, and the size of the aleurioconidia was 11.1 × 10.8 μm. For precise identification, molecular phylogenetic analysis was performed using the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF1-α), small subunit of nuclear ribosomal RNA (SSU), and RNA polymerase II subunit 1 (RPB1) gene sequences. The overall analysis confirmed that ARI-24-A5 belongs to the genus Ambrosiella, which is known for its symbiotic relationship with ambrosia beetles. In the phylogenetic tree, ARI-24-A5 shared the same taxonomic position as A. catenulata and its morphological characteristics were consistent with those of this species. Therefore, ARI-24-A5 was identified as A. catenulata, making this the first record of this species in South Korea.
KW - Ambrosia beetle
KW - Ambrosiella catenulata
KW - Korean apple orchard
KW - Symbiotic fungi
UR - http://www.scopus.com/inward/record.url?scp=85214201782&partnerID=8YFLogxK
U2 - 10.4489/kjm.520416
DO - 10.4489/kjm.520416
M3 - Article
AN - SCOPUS:85214201782
SN - 0253-651X
VL - 52
SP - 381
EP - 389
JO - Korean Journal of Mycology
JF - Korean Journal of Mycology
IS - 4
ER -