TY - JOUR
T1 - An advanced method for the determination of carboxyl methyl esterase activity using gas chromatography-chemical ionization-mass spectrometry
AU - Koo, Yeon Jong
AU - Yoon, Eunsil
AU - Song, Jong Tae
AU - Seo, Hak Soo
AU - Kim, Jeong Han
AU - Lee, Yin Won
AU - Lee, Jong Seob
AU - Cheong, Jong Joo
AU - Choi, Yang Do
PY - 2008/2/15
Y1 - 2008/2/15
N2 - We developed a quantitative method for the determination of methyl esterase activity, analyzing substrate specificity against three major signal molecules, jasmonic acid methyl ester (MeJA), salicylic acid methyl ester (MeSA), and indole-3-acetic acid methyl ester (MeIAA). We used a silylation reagent for chemical derivatization and used gas chromatography (GC)-mass spectroscopy in analyses, for high precision. To test this method, an Arabidopsis esterase gene, AtME8, was expressed in Escherichia coli, and then the kinetic parameters of the recombinant enzyme were determined for three substrates. Finally, this method was also applied to the direct quantification of phytohormones in petals from lilies and roses.
AB - We developed a quantitative method for the determination of methyl esterase activity, analyzing substrate specificity against three major signal molecules, jasmonic acid methyl ester (MeJA), salicylic acid methyl ester (MeSA), and indole-3-acetic acid methyl ester (MeIAA). We used a silylation reagent for chemical derivatization and used gas chromatography (GC)-mass spectroscopy in analyses, for high precision. To test this method, an Arabidopsis esterase gene, AtME8, was expressed in Escherichia coli, and then the kinetic parameters of the recombinant enzyme were determined for three substrates. Finally, this method was also applied to the direct quantification of phytohormones in petals from lilies and roses.
KW - Carboxyl methyl esterase
KW - Gas chromatography-mass spectroscopy
KW - Indole 3-acetic acid methyl ester
KW - Jasmonic acid methyl ester
KW - Salicylic acid methyl ester
UR - http://www.scopus.com/inward/record.url?scp=39149095591&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2008.01.015
DO - 10.1016/j.jchromb.2008.01.015
M3 - Article
C2 - 18255361
AN - SCOPUS:39149095591
SN - 1570-0232
VL - 863
SP - 80
EP - 87
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -