Abstract
We investigated anti-cancer and anti-proliferative activity of alliein and analyzed global gene expression changes by allicin treatment in human colorectal HCT116 cells. As a result, allicin decreased cell viabilities in a dose and time-dependent manner and induced apoptosis. Oligo DNA microarray analysis, we found that 7,840 genes were up-regulated more than 2-folds, whereas 10,010 genes were down-regulated more than 2-folds by 50 μM allicin treatment. To confirm specific gene expressions, we performed RT-PCR. Consistent with the results of DNA microarray analysis, allicin dramatically induced ATF3 and NAG1 gene expression. Interestingly, NAG-1 protein expression was dependent on p53 presence. Taken together, our present results increase the knowledge of the molecular mechanism of anti-cancer and anti-proliferative activity mediated by allciin in human colorectal cancer cell.
| Original language | English |
|---|---|
| Pages (from-to) | 442-447 |
| Number of pages | 6 |
| Journal | Microbiology and Biotechnology Letters |
| Volume | 38 |
| Issue number | 4 |
| State | Published - Dec 2010 |
Keywords
- Allicin
- ATF3
- Colorectal cancer
- Gene expression
- Oligo dna microarray
