Analysis of human serum amyloid A-1 concentrations using a lateral flow immunoassay with CdSe/ZnS quantum dots

Aidil Fajri, Eunseo Goh, Sanghyuk Lee, Hye Jin Lee

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A lateral flow immunoassay platform utilizing antibody functionalized water soluble CdSe/ZnS semiconductor quantum dots (QDs) was developed for the analysis of human serum amyloid A-1 (hSAA1) in a buffer solution. hSAA1 was chosen as a target protein because it is regarded as a potential biomarker associated with early diagnosis and prognosis in patients of lung cancer. The immunoassay strip on a nitrocellulose membrane was fabricated by spraying two lines composed of a test line with a monoclonal antibody against hSAA1 (10G1) (anti hSAA1) and a control line of anti-chicken IgY. While the CdSe/ZnS QDs synthesized in an organic phase were transferred to a water phase by ligand exchange using carboxylic acid modified alkane thiol. The QDs was then conjugated to monoclonal antibody against hSAA1 (14F8) [anti hSAA1 (14F8)] and used as a fluorescent detection probe. The sequential lateral flow of hSAA1 in different concentration and QDs-anti hSAA1 (14F8) complex allowed to form the surface sandwich complex of anti hSAA1 (10G1)/hSAA1/QD-anti hSAA1 (14F8), which was then analyzed using fluorescence microscope. A 100 nM concentration of hSAA1 protein can be detected by naked eyes under an optimized lateral flow buffer condition with a sensing time of 5 mins.

Original languageEnglish
Pages (from-to)429-434
Number of pages6
JournalApplied Chemistry for Engineering
Volume30
Issue number4
DOIs
StatePublished - Aug 2019

Keywords

  • CdSe/ZnS semiconductor quantum dots
  • Human serum amyloid A-1
  • Lateral flow immunoassay
  • Lung cancer Biomarker

Fingerprint

Dive into the research topics of 'Analysis of human serum amyloid A-1 concentrations using a lateral flow immunoassay with CdSe/ZnS quantum dots'. Together they form a unique fingerprint.

Cite this