Analysis of polyethoxylated ascorbic acid using spectrophotometry

Zhexue Wu; Kwang Hyeon Liu

doi:10.3839/jabc.2016.061

Analysis of polyethoxylated ascorbic acid using spectrophotometry

Zhexue Wu, Kwang Hyeon Liu

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We developed a spectrophotometric assay method for polyethoxylated ascorbic acidusing 3-ethyl ascorbic acid as standard material. The analytical method was validated by linearity, accuracy, precision, and stability. The coefficient of variation of the precision of the assay was less than 3.4%. The linearity of the calibration curves in the desired concentration range is good (r² >0.998). 3-Ethyl ascorbic acid and polyethoxylated ascorbic acid were stable in stock solution at room temperature for up to at least 6 h. The developed assay could be used for the content analysis of polyethoxylated ascorbic acid in samples.

Original language	English
Pages (from-to)	361-364
Number of pages	4
Journal	Journal of Applied Biological Chemistry
Volume	59
Issue number	4
DOIs	https://doi.org/10.3839/jabc.2016.061
State	Published - Dec 2016

Keywords

Accuracy
Polyethoxylatedascorbic acid
Precision
Spectrophotometry
Validation

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10.3839/jabc.2016.061

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title = "Analysis of polyethoxylated ascorbic acid using spectrophotometry",

abstract = "We developed a spectrophotometric assay method for polyethoxylated ascorbic acidusing 3-ethyl ascorbic acid as standard material. The analytical method was validated by linearity, accuracy, precision, and stability. The coefficient of variation of the precision of the assay was less than 3.4%. The linearity of the calibration curves in the desired concentration range is good (r2 >0.998). 3-Ethyl ascorbic acid and polyethoxylated ascorbic acid were stable in stock solution at room temperature for up to at least 6 h. The developed assay could be used for the content analysis of polyethoxylated ascorbic acid in samples.",

keywords = "Accuracy, Polyethoxylatedascorbic acid, Precision, Spectrophotometry, Validation",

author = "Zhexue Wu and Liu, {Kwang Hyeon}",

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year = "2016",

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AU - Wu, Zhexue

AU - Liu, Kwang Hyeon

N1 - Publisher Copyright: © The Korean Society for Applied Biological Chemistry 2016.

PY - 2016/12

Y1 - 2016/12

N2 - We developed a spectrophotometric assay method for polyethoxylated ascorbic acidusing 3-ethyl ascorbic acid as standard material. The analytical method was validated by linearity, accuracy, precision, and stability. The coefficient of variation of the precision of the assay was less than 3.4%. The linearity of the calibration curves in the desired concentration range is good (r2 >0.998). 3-Ethyl ascorbic acid and polyethoxylated ascorbic acid were stable in stock solution at room temperature for up to at least 6 h. The developed assay could be used for the content analysis of polyethoxylated ascorbic acid in samples.

AB - We developed a spectrophotometric assay method for polyethoxylated ascorbic acidusing 3-ethyl ascorbic acid as standard material. The analytical method was validated by linearity, accuracy, precision, and stability. The coefficient of variation of the precision of the assay was less than 3.4%. The linearity of the calibration curves in the desired concentration range is good (r2 >0.998). 3-Ethyl ascorbic acid and polyethoxylated ascorbic acid were stable in stock solution at room temperature for up to at least 6 h. The developed assay could be used for the content analysis of polyethoxylated ascorbic acid in samples.

KW - Accuracy

KW - Polyethoxylatedascorbic acid

KW - Precision

KW - Spectrophotometry

KW - Validation

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SP - 361

EP - 364

JO - Journal of Applied Biological Chemistry

JF - Journal of Applied Biological Chemistry

IS - 4

ER -

Analysis of polyethoxylated ascorbic acid using spectrophotometry

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Keywords

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