Analysis of the soluble human tooth proteome and its ability to induce dentin/tooth regeneration

So Young Chun; Hyo Jung Lee; Young Ae Choi; Kyung Min Kim; Sang Heum Baek; Hyo Sang Park; Jae Young Kim; Jung Mo Ahn; Je Yeol Cho; Dong Woo Cho; Hong In Shin; Eui Kyun Park

doi:10.1089/ten.tea.2010.0121

Analysis of the soluble human tooth proteome and its ability to induce dentin/tooth regeneration

So Young Chun, Hyo Jung Lee, Young Ae Choi, Kyung Min Kim, Sang Heum Baek, Hyo Sang Park, Jae Young Kim, Jung Mo Ahn, Je Yeol Cho, Dong Woo Cho, Hong In Shin, Eui Kyun Park

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

While the soluble proteins of human teeth consist of various extracellular matrix and bioactive proteins, they have not yet been characterized fully. Moreover, the role they play in tooth regeneration is not clear. Analysis of the soluble proteins in human teeth by liquid chromatography-mass spectrometry revealed 147 different ethylenediaminetetraacetic acid-soluble tooth proteins (ESTPs). Of these, 29 had not been shown previously to be present in human teeth. To determine their effect on the in vitro responses of dental pulp stem cells (DPSCs), DPSCs were cultured in ESTP-coated culture plates and three-dimensional scaffolds. The ESTPs significantly enhanced DPSC odontoblast differentiation and mineralization in vitro, but had only partial effect on bone marrow stem cells or adipose tissue stem cells. To test the effect of ESTPs on in vivo dentin and tooth formation, mouse embryonic tooth-forming primordia and xenogenic murine apical bud epithelium/human DPSC composites were treated with ESTPs before implantation under the renal capsule of ICR mice. ESTP treatment promoted the formation of morphologically normal teeth by the tooth-forming primordium regions and enhanced the development of a regular and large dentin structure by the composites. These observations suggest that human ESTPs contain dentinogenic proteins and can promote dentin and tooth formation.

Original language	English
Pages (from-to)	181-191
Number of pages	11
Journal	Tissue Engineering - Part A.
Volume	17
Issue number	1-2
DOIs	https://doi.org/10.1089/ten.tea.2010.0121
State	Published - 1 Jan 2011

Access to Document

10.1089/ten.tea.2010.0121

Cite this

@article{3f0834b707524b79a39086a8f3497fa5,

title = "Analysis of the soluble human tooth proteome and its ability to induce dentin/tooth regeneration",

abstract = "While the soluble proteins of human teeth consist of various extracellular matrix and bioactive proteins, they have not yet been characterized fully. Moreover, the role they play in tooth regeneration is not clear. Analysis of the soluble proteins in human teeth by liquid chromatography-mass spectrometry revealed 147 different ethylenediaminetetraacetic acid-soluble tooth proteins (ESTPs). Of these, 29 had not been shown previously to be present in human teeth. To determine their effect on the in vitro responses of dental pulp stem cells (DPSCs), DPSCs were cultured in ESTP-coated culture plates and three-dimensional scaffolds. The ESTPs significantly enhanced DPSC odontoblast differentiation and mineralization in vitro, but had only partial effect on bone marrow stem cells or adipose tissue stem cells. To test the effect of ESTPs on in vivo dentin and tooth formation, mouse embryonic tooth-forming primordia and xenogenic murine apical bud epithelium/human DPSC composites were treated with ESTPs before implantation under the renal capsule of ICR mice. ESTP treatment promoted the formation of morphologically normal teeth by the tooth-forming primordium regions and enhanced the development of a regular and large dentin structure by the composites. These observations suggest that human ESTPs contain dentinogenic proteins and can promote dentin and tooth formation.",

author = "Chun, {So Young} and Lee, {Hyo Jung} and Choi, {Young Ae} and Kim, {Kyung Min} and Baek, {Sang Heum} and Park, {Hyo Sang} and Kim, {Jae Young} and Ahn, {Jung Mo} and Cho, {Je Yeol} and Cho, {Dong Woo} and Shin, {Hong In} and Park, {Eui Kyun}",

year = "2011",

month = jan,

day = "1",

doi = "10.1089/ten.tea.2010.0121",

language = "English",

volume = "17",

pages = "181--191",

journal = "Tissue Engineering - Part A.",

issn = "1937-3341",

publisher = "Mary Ann Liebert Inc.",

number = "1-2",

}

TY - JOUR

T1 - Analysis of the soluble human tooth proteome and its ability to induce dentin/tooth regeneration

AU - Chun, So Young

AU - Lee, Hyo Jung

AU - Choi, Young Ae

AU - Kim, Kyung Min

AU - Baek, Sang Heum

AU - Park, Hyo Sang

AU - Kim, Jae Young

AU - Ahn, Jung Mo

AU - Cho, Je Yeol

AU - Cho, Dong Woo

AU - Shin, Hong In

AU - Park, Eui Kyun

PY - 2011/1/1

Y1 - 2011/1/1

N2 - While the soluble proteins of human teeth consist of various extracellular matrix and bioactive proteins, they have not yet been characterized fully. Moreover, the role they play in tooth regeneration is not clear. Analysis of the soluble proteins in human teeth by liquid chromatography-mass spectrometry revealed 147 different ethylenediaminetetraacetic acid-soluble tooth proteins (ESTPs). Of these, 29 had not been shown previously to be present in human teeth. To determine their effect on the in vitro responses of dental pulp stem cells (DPSCs), DPSCs were cultured in ESTP-coated culture plates and three-dimensional scaffolds. The ESTPs significantly enhanced DPSC odontoblast differentiation and mineralization in vitro, but had only partial effect on bone marrow stem cells or adipose tissue stem cells. To test the effect of ESTPs on in vivo dentin and tooth formation, mouse embryonic tooth-forming primordia and xenogenic murine apical bud epithelium/human DPSC composites were treated with ESTPs before implantation under the renal capsule of ICR mice. ESTP treatment promoted the formation of morphologically normal teeth by the tooth-forming primordium regions and enhanced the development of a regular and large dentin structure by the composites. These observations suggest that human ESTPs contain dentinogenic proteins and can promote dentin and tooth formation.

AB - While the soluble proteins of human teeth consist of various extracellular matrix and bioactive proteins, they have not yet been characterized fully. Moreover, the role they play in tooth regeneration is not clear. Analysis of the soluble proteins in human teeth by liquid chromatography-mass spectrometry revealed 147 different ethylenediaminetetraacetic acid-soluble tooth proteins (ESTPs). Of these, 29 had not been shown previously to be present in human teeth. To determine their effect on the in vitro responses of dental pulp stem cells (DPSCs), DPSCs were cultured in ESTP-coated culture plates and three-dimensional scaffolds. The ESTPs significantly enhanced DPSC odontoblast differentiation and mineralization in vitro, but had only partial effect on bone marrow stem cells or adipose tissue stem cells. To test the effect of ESTPs on in vivo dentin and tooth formation, mouse embryonic tooth-forming primordia and xenogenic murine apical bud epithelium/human DPSC composites were treated with ESTPs before implantation under the renal capsule of ICR mice. ESTP treatment promoted the formation of morphologically normal teeth by the tooth-forming primordium regions and enhanced the development of a regular and large dentin structure by the composites. These observations suggest that human ESTPs contain dentinogenic proteins and can promote dentin and tooth formation.

UR - http://www.scopus.com/inward/record.url?scp=78650853308&partnerID=8YFLogxK

U2 - 10.1089/ten.tea.2010.0121

DO - 10.1089/ten.tea.2010.0121

M3 - Article

C2 - 20695775

AN - SCOPUS:78650853308

SN - 1937-3341

VL - 17

SP - 181

EP - 191

JO - Tissue Engineering - Part A.

JF - Tissue Engineering - Part A.

IS - 1-2

ER -

Analysis of the soluble human tooth proteome and its ability to induce dentin/tooth regeneration

Abstract

Access to Document

Other files and links

Fingerprint

Cite this