Analysis of translocations that involve the NUP98 gene in patients with 11p15 chromosomal rearrangements

Yuri N. Kobzev; Jose Martinez-Climent; Sanggyu Lee; Jianjun Chen; Janet D. Rowley

doi:10.1002/gcc.20092

Analysis of translocations that involve the NUP98 gene in patients with 11p15 chromosomal rearrangements

Yuri N. Kobzev, Jose Martinez-Climent, Sanggyu Lee, Jianjun Chen, Janet D. Rowley

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Abstract

The NUP98 gene has been reported to be fused with at least 15 partner genes in leukemias with 11 p15 translocations. We report the results of screening of cases with cytogenetically documented rearrangements of 11p15 and the subsequent identification of involvement of NUP98 and its partner genes. We identified 49 samples from 46 hematology patients with 11p15 (including a few with 11p14) abnormalities, and using fluorescence in situ hybridization (FISH), we found that NUP98 was disrupted in 7 cases. With the use of gene-specific FISH probes, in 6 cases, we identified the partner genes, which were PRRX1 (PMX1; in 2 cases), HOXD13, KAP1GDS1, HOXC13, and TOP1. In the 3 cases for which RNA was available, RT-PCR was performed, which confirmed the FISH results and identified the location of the breakpoints in patient cDNA. Our data confirm the previous findings that NUP98 is a recurrent target in various types of leukemia.

Original language	English
Pages (from-to)	339-352
Number of pages	14
Journal	Genes Chromosomes and Cancer
Volume	41
Issue number	4
DOIs	https://doi.org/10.1002/gcc.20092
State	Published - Dec 2004

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title = "Analysis of translocations that involve the NUP98 gene in patients with 11p15 chromosomal rearrangements",

abstract = "The NUP98 gene has been reported to be fused with at least 15 partner genes in leukemias with 11 p15 translocations. We report the results of screening of cases with cytogenetically documented rearrangements of 11p15 and the subsequent identification of involvement of NUP98 and its partner genes. We identified 49 samples from 46 hematology patients with 11p15 (including a few with 11p14) abnormalities, and using fluorescence in situ hybridization (FISH), we found that NUP98 was disrupted in 7 cases. With the use of gene-specific FISH probes, in 6 cases, we identified the partner genes, which were PRRX1 (PMX1; in 2 cases), HOXD13, KAP1GDS1, HOXC13, and TOP1. In the 3 cases for which RNA was available, RT-PCR was performed, which confirmed the FISH results and identified the location of the breakpoints in patient cDNA. Our data confirm the previous findings that NUP98 is a recurrent target in various types of leukemia.",

author = "Kobzev, {Yuri N.} and Jose Martinez-Climent and Sanggyu Lee and Jianjun Chen and Rowley, {Janet D.}",

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AU - Kobzev, Yuri N.

AU - Martinez-Climent, Jose

AU - Lee, Sanggyu

AU - Chen, Jianjun

AU - Rowley, Janet D.

PY - 2004/12

Y1 - 2004/12

N2 - The NUP98 gene has been reported to be fused with at least 15 partner genes in leukemias with 11 p15 translocations. We report the results of screening of cases with cytogenetically documented rearrangements of 11p15 and the subsequent identification of involvement of NUP98 and its partner genes. We identified 49 samples from 46 hematology patients with 11p15 (including a few with 11p14) abnormalities, and using fluorescence in situ hybridization (FISH), we found that NUP98 was disrupted in 7 cases. With the use of gene-specific FISH probes, in 6 cases, we identified the partner genes, which were PRRX1 (PMX1; in 2 cases), HOXD13, KAP1GDS1, HOXC13, and TOP1. In the 3 cases for which RNA was available, RT-PCR was performed, which confirmed the FISH results and identified the location of the breakpoints in patient cDNA. Our data confirm the previous findings that NUP98 is a recurrent target in various types of leukemia.

AB - The NUP98 gene has been reported to be fused with at least 15 partner genes in leukemias with 11 p15 translocations. We report the results of screening of cases with cytogenetically documented rearrangements of 11p15 and the subsequent identification of involvement of NUP98 and its partner genes. We identified 49 samples from 46 hematology patients with 11p15 (including a few with 11p14) abnormalities, and using fluorescence in situ hybridization (FISH), we found that NUP98 was disrupted in 7 cases. With the use of gene-specific FISH probes, in 6 cases, we identified the partner genes, which were PRRX1 (PMX1; in 2 cases), HOXD13, KAP1GDS1, HOXC13, and TOP1. In the 3 cases for which RNA was available, RT-PCR was performed, which confirmed the FISH results and identified the location of the breakpoints in patient cDNA. Our data confirm the previous findings that NUP98 is a recurrent target in various types of leukemia.

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JO - Genes Chromosomes and Cancer

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Analysis of translocations that involve the NUP98 gene in patients with 11p15 chromosomal rearrangements

Abstract

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