TY - JOUR
T1 - Anti-inflammatory activity of compounds from the rhizome of Cnidium officinale
AU - Tran, Huynh Nguyen Khanh
AU - Cao, Thao Quyen
AU - Kim, Jeong Ah
AU - Youn, Ui Joung
AU - Kim, Sanghee
AU - Woo, Mi Hee
AU - Min, Byung Sun
N1 - Publisher Copyright:
© 2018, The Pharmaceutical Society of Korea.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Five new compounds, 9,3′-dimethoxyhierochin A (1), 6-oxo-trans-neocnidilide (2), (±)-(3E)-trans-6-hydroxy-7-methoxydihydroligustilide (3), (±)-cnidiumin (4), and 6-(1-oxopentyl)-salicylic acid methyl ester (5), together with twenty known compounds (6–25), were isolated from the rhizome of Cnidium officinale. The chemical structures of new compounds were established by NMR spectroscopic techniques, mass spectrometry, Mosher’s method, and CD spectrum. Their anti-inflammatory activities were evaluated against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW 264.7 cells. Compounds 7, 13, and 14 showed inhibitory effects with IC50 values of 5.1, 24.5, and 27.8 μM, respectively. In addition, compounds 7, 13, and 14 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression and cyclooxygenase-2 (COX-2) protein in a concentration-dependent manner.
AB - Five new compounds, 9,3′-dimethoxyhierochin A (1), 6-oxo-trans-neocnidilide (2), (±)-(3E)-trans-6-hydroxy-7-methoxydihydroligustilide (3), (±)-cnidiumin (4), and 6-(1-oxopentyl)-salicylic acid methyl ester (5), together with twenty known compounds (6–25), were isolated from the rhizome of Cnidium officinale. The chemical structures of new compounds were established by NMR spectroscopic techniques, mass spectrometry, Mosher’s method, and CD spectrum. Their anti-inflammatory activities were evaluated against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW 264.7 cells. Compounds 7, 13, and 14 showed inhibitory effects with IC50 values of 5.1, 24.5, and 27.8 μM, respectively. In addition, compounds 7, 13, and 14 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression and cyclooxygenase-2 (COX-2) protein in a concentration-dependent manner.
KW - Anti-inflammatory
KW - Cnidium officinale
KW - NO inhibition
KW - Umbelliferae
UR - http://www.scopus.com/inward/record.url?scp=85049154377&partnerID=8YFLogxK
U2 - 10.1007/s12272-018-1048-9
DO - 10.1007/s12272-018-1048-9
M3 - Article
C2 - 29961195
AN - SCOPUS:85049154377
SN - 0253-6269
VL - 41
SP - 977
EP - 985
JO - Archives of Pharmacal Research
JF - Archives of Pharmacal Research
IS - 10
ER -