Abstract
The present study investigated the anti-inflammatory effects of dichloromethane extract of Auriculariaauricula-judae. Dichloromethane extract of Auricularia auricula-judae inhibited Lipopolysaccharide(LPS)-induced nitric oxide (NO) production significantly in a dose-dependent manner in the concentration ≥10 μg/ml (p < 0.05). Furthermore, RT-PCR results of this study indicated that the extract markedly reducedthe expressions of inflammatory cytokines (IL-6, TNF-α and IL-1β) mRNA in LPS-treated murine RAW264.7 macrophages, which could possibly ameliorate the inflammation. Nevertheless, dichloromethaneextract of Auricularia auricula-judae did not show complete inhibition of IL-6 mRNA expression. Theinhibition of IL-1β cytokine at protein level was also observed in a dose dependent manner. In conclusion,the current study revealed the previously unknown effect of dichloromethane ethyl extract of Auriculariaauricula-judae inhibitions of the production of NO, IL-6, TNF-α and IL-1β in LPS-stimulated macrophages.
| Original language | English |
|---|---|
| Pages (from-to) | 11-14 |
| Number of pages | 4 |
| Journal | Toxicological Research |
| Volume | 27 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 2011 |
Keywords
- Anti-inflammatory
- Auricularia auricula-judae
- Inflammatory cytokines
- Nitric oxide