TY - JOUR
T1 - Anti-inflammatory activity of dichloromethane extract of Auricularia auricula-judae in RAW264.7 cells
AU - Damte, Dereje
AU - Reza, Md Ahsanur
AU - Lee, Seung Jin
AU - Jo, Woo Sik
AU - Park, Seung Chun
PY - 2011/3
Y1 - 2011/3
N2 - The present study investigated the anti-inflammatory effects of dichloromethane extract of Auriculariaauricula-judae. Dichloromethane extract of Auricularia auricula-judae inhibited Lipopolysaccharide(LPS)-induced nitric oxide (NO) production significantly in a dose-dependent manner in the concentration ≥10 μg/ml (p < 0.05). Furthermore, RT-PCR results of this study indicated that the extract markedly reducedthe expressions of inflammatory cytokines (IL-6, TNF-α and IL-1β) mRNA in LPS-treated murine RAW264.7 macrophages, which could possibly ameliorate the inflammation. Nevertheless, dichloromethaneextract of Auricularia auricula-judae did not show complete inhibition of IL-6 mRNA expression. Theinhibition of IL-1β cytokine at protein level was also observed in a dose dependent manner. In conclusion,the current study revealed the previously unknown effect of dichloromethane ethyl extract of Auriculariaauricula-judae inhibitions of the production of NO, IL-6, TNF-α and IL-1β in LPS-stimulated macrophages.
AB - The present study investigated the anti-inflammatory effects of dichloromethane extract of Auriculariaauricula-judae. Dichloromethane extract of Auricularia auricula-judae inhibited Lipopolysaccharide(LPS)-induced nitric oxide (NO) production significantly in a dose-dependent manner in the concentration ≥10 μg/ml (p < 0.05). Furthermore, RT-PCR results of this study indicated that the extract markedly reducedthe expressions of inflammatory cytokines (IL-6, TNF-α and IL-1β) mRNA in LPS-treated murine RAW264.7 macrophages, which could possibly ameliorate the inflammation. Nevertheless, dichloromethaneextract of Auricularia auricula-judae did not show complete inhibition of IL-6 mRNA expression. Theinhibition of IL-1β cytokine at protein level was also observed in a dose dependent manner. In conclusion,the current study revealed the previously unknown effect of dichloromethane ethyl extract of Auriculariaauricula-judae inhibitions of the production of NO, IL-6, TNF-α and IL-1β in LPS-stimulated macrophages.
KW - Anti-inflammatory
KW - Auricularia auricula-judae
KW - Inflammatory cytokines
KW - Nitric oxide
UR - https://www.scopus.com/pages/publications/84863120254
U2 - 10.5487/TR.2011.27.1.011
DO - 10.5487/TR.2011.27.1.011
M3 - Short survey
AN - SCOPUS:84863120254
SN - 1976-8257
VL - 27
SP - 11
EP - 14
JO - Toxicological Research
JF - Toxicological Research
IS - 1
ER -