Anti-inflammatory and antioxidant effects of soroseris hirsuta extract by regulating inos/nf-κb and nrf2/ho-1 pathways in murine macrophage raw 264.7 cells

Woo Jin Lee, Wan Yi Li, Sang Woo Lee, Sung Keun Jung

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Until now, the physiological effects of Soroseris hirsuta were primarily unknown. Here we have evaluated the anti-inflammatory and antioxidant effects of Soroseris hirsuta extract (SHE) on lipopolysaccharide (LPS)-activated murine macrophages RAW 264.7 cells. SHE inhibited nitric oxide expression and inducible nitric oxide synthase expression in RAW 264.7 cells treated with LPS. Moreover, SHE suppressed LPS-induced phosphorylation of IκB kinase, inhibitor of kappa B, p65, p38, and c-JUN N-terminal kinase. Western blot and immunofluorescence analyses showed that SHE suppressed p65 nuclear translocation induced by LPS. Furthermore, SHE inhibited the reactive oxygen species in LPS-treated RAW 264.7 cells. SHE significantly increased heme oxygenase-1 expression and the nuclear translocation of nuclear factor erythroid 2-related factor 2. SHE suppressed LPS-induced interleukin-1β mRNA expression in RAW 264.7 cells. Thus, SHE is a promising nutraceutical as it displays anti-inflammatory and antioxidant properties.

Original languageEnglish
Article number4711
JournalApplied Sciences (Switzerland)
Volume11
Issue number10
DOIs
StatePublished - 2 May 2021

Keywords

  • Anti-inflammation
  • Antioxidant
  • Heme oxygenase-1
  • Nitric oxide
  • Soroseris hirsuta

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