Anti-inflammatory effect of oleuropein on microglia through regulation of Drp1-dependent mitochondrial fission

Junghyung Park; Ju Sik Min; Unbin Chae; Joon Yeop Lee; Kyung Sik Song; Hyun Shik Lee; Hong Jun Lee; Sang Rae Lee; Dong Seok Lee

doi:10.1016/j.jneuroim.2017.02.019

Anti-inflammatory effect of oleuropein on microglia through regulation of Drp1-dependent mitochondrial fission

Junghyung Park, Ju Sik Min, Unbin Chae, Joon Yeop Lee, Kyung Sik Song, Hyun Shik Lee, Hong Jun Lee, Sang Rae Lee, Dong Seok Lee

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Oleuropein is a primary phenolic compound found in olive leaf and Fraxinus rhynchophylla. Here, we investigated the impact of oleuropein on LPS-induced BV-2 microglial cells. Oleuropein suppressed the LPS-induced increase in pro-inflammatory mediators, such as nitric oxide, and pro-inflammatory cytokines, via inhibition of ERK/p38/NF-κB activation and reactive oxygen species (ROS) generation. Furthermore, it suppressed LPS-induced excessive mitochondrial fission, which regulates mitochondrial ROS generation and pro-inflammatory response by diminishing Drp1 dephosphorylation. Collectively, we demonstrated that oleuropein suppresses pro-inflammatory response of microglia by inhibiting Drp1-dependent mitochondrial fission. Our findings suggest a potential role of oleuropein in microglial inflammation-mediated neurodegenerative disorders.

Original language	English
Pages (from-to)	46-52
Number of pages	7
Journal	Journal of Neuroimmunology
Volume	306
DOIs	https://doi.org/10.1016/j.jneuroim.2017.02.019
State	Published - 15 May 2017

Keywords

Drp1
Inflammation
Microglia
Mitochondrial dynamics
Oleuropein
ROS

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10.1016/j.jneuroim.2017.02.019

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@article{2ed1781b6e28421e8714d48fa05a2099,

title = "Anti-inflammatory effect of oleuropein on microglia through regulation of Drp1-dependent mitochondrial fission",

abstract = "Oleuropein is a primary phenolic compound found in olive leaf and Fraxinus rhynchophylla. Here, we investigated the impact of oleuropein on LPS-induced BV-2 microglial cells. Oleuropein suppressed the LPS-induced increase in pro-inflammatory mediators, such as nitric oxide, and pro-inflammatory cytokines, via inhibition of ERK/p38/NF-κB activation and reactive oxygen species (ROS) generation. Furthermore, it suppressed LPS-induced excessive mitochondrial fission, which regulates mitochondrial ROS generation and pro-inflammatory response by diminishing Drp1 dephosphorylation. Collectively, we demonstrated that oleuropein suppresses pro-inflammatory response of microglia by inhibiting Drp1-dependent mitochondrial fission. Our findings suggest a potential role of oleuropein in microglial inflammation-mediated neurodegenerative disorders.",

keywords = "Drp1, Inflammation, Microglia, Mitochondrial dynamics, Oleuropein, ROS",

author = "Junghyung Park and Min, {Ju Sik} and Unbin Chae and Lee, {Joon Yeop} and Song, {Kyung Sik} and Lee, {Hyun Shik} and Lee, {Hong Jun} and Lee, {Sang Rae} and Lee, {Dong Seok}",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

year = "2017",

month = may,

day = "15",

doi = "10.1016/j.jneuroim.2017.02.019",

language = "English",

volume = "306",

pages = "46--52",

journal = "Journal of Neuroimmunology",

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TY - JOUR

T1 - Anti-inflammatory effect of oleuropein on microglia through regulation of Drp1-dependent mitochondrial fission

AU - Park, Junghyung

AU - Min, Ju Sik

AU - Chae, Unbin

AU - Lee, Joon Yeop

AU - Song, Kyung Sik

AU - Lee, Hyun Shik

AU - Lee, Hong Jun

AU - Lee, Sang Rae

AU - Lee, Dong Seok

PY - 2017/5/15

Y1 - 2017/5/15

N2 - Oleuropein is a primary phenolic compound found in olive leaf and Fraxinus rhynchophylla. Here, we investigated the impact of oleuropein on LPS-induced BV-2 microglial cells. Oleuropein suppressed the LPS-induced increase in pro-inflammatory mediators, such as nitric oxide, and pro-inflammatory cytokines, via inhibition of ERK/p38/NF-κB activation and reactive oxygen species (ROS) generation. Furthermore, it suppressed LPS-induced excessive mitochondrial fission, which regulates mitochondrial ROS generation and pro-inflammatory response by diminishing Drp1 dephosphorylation. Collectively, we demonstrated that oleuropein suppresses pro-inflammatory response of microglia by inhibiting Drp1-dependent mitochondrial fission. Our findings suggest a potential role of oleuropein in microglial inflammation-mediated neurodegenerative disorders.

AB - Oleuropein is a primary phenolic compound found in olive leaf and Fraxinus rhynchophylla. Here, we investigated the impact of oleuropein on LPS-induced BV-2 microglial cells. Oleuropein suppressed the LPS-induced increase in pro-inflammatory mediators, such as nitric oxide, and pro-inflammatory cytokines, via inhibition of ERK/p38/NF-κB activation and reactive oxygen species (ROS) generation. Furthermore, it suppressed LPS-induced excessive mitochondrial fission, which regulates mitochondrial ROS generation and pro-inflammatory response by diminishing Drp1 dephosphorylation. Collectively, we demonstrated that oleuropein suppresses pro-inflammatory response of microglia by inhibiting Drp1-dependent mitochondrial fission. Our findings suggest a potential role of oleuropein in microglial inflammation-mediated neurodegenerative disorders.

KW - Drp1

KW - Inflammation

KW - Microglia

KW - Mitochondrial dynamics

KW - Oleuropein

KW - ROS

UR - http://www.scopus.com/inward/record.url?scp=85015400482&partnerID=8YFLogxK

U2 - 10.1016/j.jneuroim.2017.02.019

DO - 10.1016/j.jneuroim.2017.02.019

M3 - Article

C2 - 28385187

AN - SCOPUS:85015400482

SN - 0165-5728

VL - 306

SP - 46

EP - 52

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

ER -

Anti-inflammatory effect of oleuropein on microglia through regulation of Drp1-dependent mitochondrial fission

Abstract

Keywords

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