Anti-inflammatory effect potentials of ethanol extracts from fermentated caryopteris incana by lactobacillus plantarum on induced to lps with raw 264.7 cell

In this study, the inflammation of ethanol extracts from Caryopteris incana (CI) and fermented C. incana (FCI) on induced to lipopolysaccharide with Raw 264.7 cell was tested. The composition profile of L. plantarum was changed by fermentation, and confirmed by HPLC analysis. We performed the 3-[4,5-dimethylthiazol]-2-yl]-2,5-diphenyltetrazolium bromide assay to evaluate the toxicity of CI and FCI extracts. In cell viability, cell toxicity was not shown at 5, 10 and 15 μg/mL of CI extracts and 10, 20, 30 and 40 μg/mL of FCI extracts. The results of inducible nitric oxide synthase and cyclooxygenase-2 protein production were confirmed to be inhibitory in a concentration-dependent manner, respectively. Additionally, protein expression of nitric oxide and prostaglandin E₂ by CI and FCI extracts were also inhibited in a concentration-dependent manner. In the result of pro-inflammatory cytokine, 15 μg/mL concentration of CI extracts was showed tumar necrosis factor (TNF)-α (57.3%), interleukin (IL)-6 (35.2%), and IL-1β (48.0%), respectively. And 40 μg/mL of FCI extracts was showed TNF-α (34.6%), IL-6 (32.1%), and IL-1β (30.0%), respectively. These results suggest that FCI extracts showed better effect of anti-inflammatory than CI extracts. Therefore, it was found that both CI and FCI can be used as an excellent material for the development of new anti-inflammatory resource.