Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages

Eun Myoung Shin; Hong Yu Zhou; Lian Yu Guo; Jeong Ah Kim; Seung Ho Lee; Irmgard Merfort; Sam Sik Kang; Hak Sung Kim; Sanghee Kim; Yeong Shik Kim

doi:10.1016/j.intimp.2008.06.008

Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages

Eun Myoung Shin, Hong Yu Zhou, Lian Yu Guo, Jeong Ah Kim, Seung Ho Lee, Irmgard Merfort, Sam Sik Kang, Hak Sung Kim, Sanghee Kim, Yeong Shik Kim

Research output: Contribution to journal › Article › peer-review

146 Scopus citations

Abstract

The anti-inflammatory effects of glycyrol, a benzofuran coumarin isolated from Glycyrrhizae Radix, were studied. Glycyrol of 5, 25 and 50 μM dose-dependently inhibited nitric oxide (NO) production by down-regulating inducible nitric oxide synthase (iNOS), and alleviated cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 macrophages, in both the mRNA and the protein. Furthermore, glycyrol dose-dependently decreased the mRNA of the pro-inflammatory cytokines IL-1β and IL-6. LPS-induced NF-κB activation was prevented in RAW264.7 macrophages by inhibition of I-κBα phosphorylation. In addition, administration of glycyrol (30 and 100 mg/kg, i.p) reduced the thickness of carrageenan-induced mouse-paw edema swelling. Taken together, our results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti-inflammatory effect is attributed to the inhibition I-κBα phosphorylation.

Original language	English
Pages (from-to)	1524-1532
Number of pages	9
Journal	International Immunopharmacology
Volume	8
Issue number	11
DOIs	https://doi.org/10.1016/j.intimp.2008.06.008
State	Published - Nov 2008

Keywords

Anti-inflammatory activity
COX-2
Glycyrol
Glycyrrhizae Radix
iNOS
NF-κB

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10.1016/j.intimp.2008.06.008

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@article{4072be03dae94b2a932bd9aa2af8a886,

title = "Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages",

abstract = "The anti-inflammatory effects of glycyrol, a benzofuran coumarin isolated from Glycyrrhizae Radix, were studied. Glycyrol of 5, 25 and 50 μM dose-dependently inhibited nitric oxide (NO) production by down-regulating inducible nitric oxide synthase (iNOS), and alleviated cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 macrophages, in both the mRNA and the protein. Furthermore, glycyrol dose-dependently decreased the mRNA of the pro-inflammatory cytokines IL-1β and IL-6. LPS-induced NF-κB activation was prevented in RAW264.7 macrophages by inhibition of I-κBα phosphorylation. In addition, administration of glycyrol (30 and 100 mg/kg, i.p) reduced the thickness of carrageenan-induced mouse-paw edema swelling. Taken together, our results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti-inflammatory effect is attributed to the inhibition I-κBα phosphorylation.",

keywords = "Anti-inflammatory activity, COX-2, Glycyrol, Glycyrrhizae Radix, iNOS, NF-κB",

author = "Shin, {Eun Myoung} and Zhou, {Hong Yu} and Guo, {Lian Yu} and Kim, {Jeong Ah} and Lee, {Seung Ho} and Irmgard Merfort and Kang, {Sam Sik} and Kim, {Hak Sung} and Sanghee Kim and Kim, {Yeong Shik}",

year = "2008",

month = nov,

doi = "10.1016/j.intimp.2008.06.008",

language = "English",

volume = "8",

pages = "1524--1532",

journal = "International Immunopharmacology",

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publisher = "Elsevier B.V.",

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T1 - Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages

AU - Shin, Eun Myoung

AU - Zhou, Hong Yu

AU - Guo, Lian Yu

AU - Kim, Jeong Ah

AU - Lee, Seung Ho

AU - Merfort, Irmgard

AU - Kang, Sam Sik

AU - Kim, Hak Sung

AU - Kim, Sanghee

AU - Kim, Yeong Shik

PY - 2008/11

Y1 - 2008/11

N2 - The anti-inflammatory effects of glycyrol, a benzofuran coumarin isolated from Glycyrrhizae Radix, were studied. Glycyrol of 5, 25 and 50 μM dose-dependently inhibited nitric oxide (NO) production by down-regulating inducible nitric oxide synthase (iNOS), and alleviated cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 macrophages, in both the mRNA and the protein. Furthermore, glycyrol dose-dependently decreased the mRNA of the pro-inflammatory cytokines IL-1β and IL-6. LPS-induced NF-κB activation was prevented in RAW264.7 macrophages by inhibition of I-κBα phosphorylation. In addition, administration of glycyrol (30 and 100 mg/kg, i.p) reduced the thickness of carrageenan-induced mouse-paw edema swelling. Taken together, our results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti-inflammatory effect is attributed to the inhibition I-κBα phosphorylation.

AB - The anti-inflammatory effects of glycyrol, a benzofuran coumarin isolated from Glycyrrhizae Radix, were studied. Glycyrol of 5, 25 and 50 μM dose-dependently inhibited nitric oxide (NO) production by down-regulating inducible nitric oxide synthase (iNOS), and alleviated cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 macrophages, in both the mRNA and the protein. Furthermore, glycyrol dose-dependently decreased the mRNA of the pro-inflammatory cytokines IL-1β and IL-6. LPS-induced NF-κB activation was prevented in RAW264.7 macrophages by inhibition of I-κBα phosphorylation. In addition, administration of glycyrol (30 and 100 mg/kg, i.p) reduced the thickness of carrageenan-induced mouse-paw edema swelling. Taken together, our results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti-inflammatory effect is attributed to the inhibition I-κBα phosphorylation.

KW - Anti-inflammatory activity

KW - COX-2

KW - Glycyrol

KW - Glycyrrhizae Radix

KW - iNOS

KW - NF-κB

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Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages

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