Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages

Eun Myoung Shin; Hong Yu Zhou; Lian Yu Guo; Jeong Ah Kim; Seung Ho Lee; Irmgard Merfort; Sam Sik Kang; Hak Sung Kim; Sanghee Kim; Yeong Shik Kim

doi:10.1016/j.intimp.2008.06.008

Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages

Eun Myoung Shin
, Hong Yu Zhou
, Lian Yu Guo
, Jeong Ah Kim
, Seung Ho Lee
, Irmgard Merfort
, Sam Sik Kang
, Hak Sung Kim
, Sanghee Kim
, Yeong Shik Kim

Seoul National University
Shenyang Pharmaceutical University
Yeungnam University
University of Freiburg
Wonkwang University

Research output: Contribution to journal › Article › peer-review

151 Scopus citations

Abstract

The anti-inflammatory effects of glycyrol, a benzofuran coumarin isolated from Glycyrrhizae Radix, were studied. Glycyrol of 5, 25 and 50 μM dose-dependently inhibited nitric oxide (NO) production by down-regulating inducible nitric oxide synthase (iNOS), and alleviated cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 macrophages, in both the mRNA and the protein. Furthermore, glycyrol dose-dependently decreased the mRNA of the pro-inflammatory cytokines IL-1β and IL-6. LPS-induced NF-κB activation was prevented in RAW264.7 macrophages by inhibition of I-κBα phosphorylation. In addition, administration of glycyrol (30 and 100 mg/kg, i.p) reduced the thickness of carrageenan-induced mouse-paw edema swelling. Taken together, our results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti-inflammatory effect is attributed to the inhibition I-κBα phosphorylation.

Original language	English
Pages (from-to)	1524-1532
Number of pages	9
Journal	International Immunopharmacology
Volume	8
Issue number	11
DOIs	https://doi.org/10.1016/j.intimp.2008.06.008
State	Published - Nov 2008

Keywords

Anti-inflammatory activity
COX-2
Glycyrol
Glycyrrhizae Radix
iNOS
NF-κB

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10.1016/j.intimp.2008.06.008

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@article{4072be03dae94b2a932bd9aa2af8a886,

title = "Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages",

abstract = "The anti-inflammatory effects of glycyrol, a benzofuran coumarin isolated from Glycyrrhizae Radix, were studied. Glycyrol of 5, 25 and 50 μM dose-dependently inhibited nitric oxide (NO) production by down-regulating inducible nitric oxide synthase (iNOS), and alleviated cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 macrophages, in both the mRNA and the protein. Furthermore, glycyrol dose-dependently decreased the mRNA of the pro-inflammatory cytokines IL-1β and IL-6. LPS-induced NF-κB activation was prevented in RAW264.7 macrophages by inhibition of I-κBα phosphorylation. In addition, administration of glycyrol (30 and 100 mg/kg, i.p) reduced the thickness of carrageenan-induced mouse-paw edema swelling. Taken together, our results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti-inflammatory effect is attributed to the inhibition I-κBα phosphorylation.",

keywords = "Anti-inflammatory activity, COX-2, Glycyrol, Glycyrrhizae Radix, iNOS, NF-κB",

author = "Shin, \{Eun Myoung\} and Zhou, \{Hong Yu\} and Guo, \{Lian Yu\} and Kim, \{Jeong Ah\} and Lee, \{Seung Ho\} and Irmgard Merfort and Kang, \{Sam Sik\} and Kim, \{Hak Sung\} and Sanghee Kim and Kim, \{Yeong Shik\}",

year = "2008",

month = nov,

doi = "10.1016/j.intimp.2008.06.008",

language = "English",

volume = "8",

pages = "1524--1532",

journal = "International Immunopharmacology",

issn = "1567-5769",

publisher = "Elsevier B.V.",

number = "11",

}

TY - JOUR

T1 - Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages

AU - Shin, Eun Myoung

AU - Zhou, Hong Yu

AU - Guo, Lian Yu

AU - Kim, Jeong Ah

AU - Lee, Seung Ho

AU - Merfort, Irmgard

AU - Kang, Sam Sik

AU - Kim, Hak Sung

AU - Kim, Sanghee

AU - Kim, Yeong Shik

PY - 2008/11

Y1 - 2008/11

N2 - The anti-inflammatory effects of glycyrol, a benzofuran coumarin isolated from Glycyrrhizae Radix, were studied. Glycyrol of 5, 25 and 50 μM dose-dependently inhibited nitric oxide (NO) production by down-regulating inducible nitric oxide synthase (iNOS), and alleviated cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 macrophages, in both the mRNA and the protein. Furthermore, glycyrol dose-dependently decreased the mRNA of the pro-inflammatory cytokines IL-1β and IL-6. LPS-induced NF-κB activation was prevented in RAW264.7 macrophages by inhibition of I-κBα phosphorylation. In addition, administration of glycyrol (30 and 100 mg/kg, i.p) reduced the thickness of carrageenan-induced mouse-paw edema swelling. Taken together, our results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti-inflammatory effect is attributed to the inhibition I-κBα phosphorylation.

AB - The anti-inflammatory effects of glycyrol, a benzofuran coumarin isolated from Glycyrrhizae Radix, were studied. Glycyrol of 5, 25 and 50 μM dose-dependently inhibited nitric oxide (NO) production by down-regulating inducible nitric oxide synthase (iNOS), and alleviated cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW264.7 macrophages, in both the mRNA and the protein. Furthermore, glycyrol dose-dependently decreased the mRNA of the pro-inflammatory cytokines IL-1β and IL-6. LPS-induced NF-κB activation was prevented in RAW264.7 macrophages by inhibition of I-κBα phosphorylation. In addition, administration of glycyrol (30 and 100 mg/kg, i.p) reduced the thickness of carrageenan-induced mouse-paw edema swelling. Taken together, our results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti-inflammatory effect is attributed to the inhibition I-κBα phosphorylation.

KW - Anti-inflammatory activity

KW - COX-2

KW - Glycyrol

KW - Glycyrrhizae Radix

KW - iNOS

KW - NF-κB

UR - https://www.scopus.com/pages/publications/51749117556

U2 - 10.1016/j.intimp.2008.06.008

DO - 10.1016/j.intimp.2008.06.008

M3 - Article

C2 - 18621150

AN - SCOPUS:51749117556

SN - 1567-5769

VL - 8

SP - 1524

EP - 1532

JO - International Immunopharmacology

JF - International Immunopharmacology

IS - 11

ER -

Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages

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Keywords

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