Anti-inflammatory Potential of Saponins from Aster tataricus via NF-κB/MAPK Activation

Xiang Dong Su; Hyun Jae Jang; Cai Yi Wang; Seung Woong Lee; Mun Chual Rho; Young Ho Kim; Seo Young Yang

doi:10.1021/acs.jnatprod.8b00856

Anti-inflammatory Potential of Saponins from Aster tataricus via NF-κB/MAPK Activation

Xiang Dong Su, Hyun Jae Jang, Cai Yi Wang, Seung Woong Lee, Mun Chual Rho, Young Ho Kim, Seo Young Yang

Department of Biology Education

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Four new aster saponins (1-4) together with five known analogues (5-9) were isolated from Aster tataricus. The chemical structures of 1-4 were elucidated based on spectrometric and spectroscopic analysis and comparison with reported data. The potential anti-inflammatory activities of aster saponins 1-9 were evaluated subsequently by measuring lipopolysaccharide (LPS)-enhanced nitric oxide (NO) formation in murine macrophages. Among these, aster saponin B (6) exhibited the most potent inhibitory activity (IC₅₀: 1.2 μM). Additionally, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein levels were dose-dependently suppressed by 6 in LPS-activated RAW 264.7 cells. Investigation of the anti-inflammatory mechanism indicated that 6 attenuated the phosphorylation and degradation of the inhibitor of NF-κB (IκB), which led to the blocking of NF-κB p65 translocation to the nucleus.

Original language	English
Pages (from-to)	1139-1148
Number of pages	10
Journal	Journal of Natural Products
Volume	82
Issue number	5
DOIs	https://doi.org/10.1021/acs.jnatprod.8b00856
State	Published - 24 May 2019

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title = "Anti-inflammatory Potential of Saponins from Aster tataricus via NF-κB/MAPK Activation",

abstract = "Four new aster saponins (1-4) together with five known analogues (5-9) were isolated from Aster tataricus. The chemical structures of 1-4 were elucidated based on spectrometric and spectroscopic analysis and comparison with reported data. The potential anti-inflammatory activities of aster saponins 1-9 were evaluated subsequently by measuring lipopolysaccharide (LPS)-enhanced nitric oxide (NO) formation in murine macrophages. Among these, aster saponin B (6) exhibited the most potent inhibitory activity (IC50: 1.2 μM). Additionally, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein levels were dose-dependently suppressed by 6 in LPS-activated RAW 264.7 cells. Investigation of the anti-inflammatory mechanism indicated that 6 attenuated the phosphorylation and degradation of the inhibitor of NF-κB (IκB), which led to the blocking of NF-κB p65 translocation to the nucleus.",

author = "Su, {Xiang Dong} and Jang, {Hyun Jae} and Wang, {Cai Yi} and Lee, {Seung Woong} and Rho, {Mun Chual} and Kim, {Young Ho} and Yang, {Seo Young}",

note = "Publisher Copyright: {\textcopyright} 2019 American Chemical Society and American Society of Pharmacognosy.",

year = "2019",

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doi = "10.1021/acs.jnatprod.8b00856",

language = "English",

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T1 - Anti-inflammatory Potential of Saponins from Aster tataricus via NF-κB/MAPK Activation

AU - Su, Xiang Dong

AU - Jang, Hyun Jae

AU - Wang, Cai Yi

AU - Lee, Seung Woong

AU - Rho, Mun Chual

AU - Kim, Young Ho

AU - Yang, Seo Young

PY - 2019/5/24

Y1 - 2019/5/24

N2 - Four new aster saponins (1-4) together with five known analogues (5-9) were isolated from Aster tataricus. The chemical structures of 1-4 were elucidated based on spectrometric and spectroscopic analysis and comparison with reported data. The potential anti-inflammatory activities of aster saponins 1-9 were evaluated subsequently by measuring lipopolysaccharide (LPS)-enhanced nitric oxide (NO) formation in murine macrophages. Among these, aster saponin B (6) exhibited the most potent inhibitory activity (IC50: 1.2 μM). Additionally, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein levels were dose-dependently suppressed by 6 in LPS-activated RAW 264.7 cells. Investigation of the anti-inflammatory mechanism indicated that 6 attenuated the phosphorylation and degradation of the inhibitor of NF-κB (IκB), which led to the blocking of NF-κB p65 translocation to the nucleus.

AB - Four new aster saponins (1-4) together with five known analogues (5-9) were isolated from Aster tataricus. The chemical structures of 1-4 were elucidated based on spectrometric and spectroscopic analysis and comparison with reported data. The potential anti-inflammatory activities of aster saponins 1-9 were evaluated subsequently by measuring lipopolysaccharide (LPS)-enhanced nitric oxide (NO) formation in murine macrophages. Among these, aster saponin B (6) exhibited the most potent inhibitory activity (IC50: 1.2 μM). Additionally, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein levels were dose-dependently suppressed by 6 in LPS-activated RAW 264.7 cells. Investigation of the anti-inflammatory mechanism indicated that 6 attenuated the phosphorylation and degradation of the inhibitor of NF-κB (IκB), which led to the blocking of NF-κB p65 translocation to the nucleus.

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JF - Journal of Natural Products

IS - 5

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Anti-inflammatory Potential of Saponins from Aster tataricus via NF-κB/MAPK Activation

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