Anti-osteoporotic activities of fucosterol from sea mustard (Undaria pinnatifida)

Myun Ho Bang; Hong Hee Kim; Dae Young Lee; Min Woo Han; Yoon Su Baek; Dae Kyun Chung; Nam In Baek

doi:10.1007/s10068-011-0048-z

Anti-osteoporotic activities of fucosterol from sea mustard (Undaria pinnatifida)

Myun Ho Bang, Hong Hee Kim, Dae Young Lee, Min Woo Han, Yoon Su Baek, Dae Kyun Chung, Nam In Baek

School of Life Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Samples of the edible seaweed, sea mustard (Undaria pinnatifida) were extracted with 80% aqueous methanol, and the concentrated extract was partitioned with ethyl acetate, n-butanol, and water, successively. Isolated fucosterol (1) from the ethyl acetate layers using repeated silica gel and octadecyl silica gel (C₁₈, ODS-A) column chromatography and identified it using spectroscopic data including NMR, MS, and infrared spectroscopy (IR). Osteoclasts are specialized multinucleated cells with bone resorbing activity. The induction of c-Fos and NFATc1 transcription factors by receptor activator of nuclear factor κB ligand (RANKL) is a key event in osteoclast differentiation. Isolated and identification compound 1 was investigated in vitro for their anti-osteoclastic activity. Compound 1 inhibited osteoclastic differentiation in a dose-dependent manner without cytotoxicity. In addition, the inhibitory mechanisms of compound 1 were investigated. Compound 1 induced a clear reduction in the expressions of NFATc1 and c-Fos upon RANKL stimulation. In summary, compound 1 from sea mustard showed antiosteoporotic potential.

Original language	English
Pages (from-to)	343-347
Number of pages	5
Journal	Food Science and Biotechnology
Volume	20
Issue number	2
DOIs	https://doi.org/10.1007/s10068-011-0048-z
State	Published - Apr 2011

Keywords

Anti-osteoporosis
Fucosterol
Nuclear factor of activated T cells c1 (NFATc1)
Receptor activator of nuclear factor κB ligand (RANKL)
Sea mustard
Undaria pinnatifida

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title = "Anti-osteoporotic activities of fucosterol from sea mustard (Undaria pinnatifida)",

abstract = "Samples of the edible seaweed, sea mustard (Undaria pinnatifida) were extracted with 80% aqueous methanol, and the concentrated extract was partitioned with ethyl acetate, n-butanol, and water, successively. Isolated fucosterol (1) from the ethyl acetate layers using repeated silica gel and octadecyl silica gel (C18, ODS-A) column chromatography and identified it using spectroscopic data including NMR, MS, and infrared spectroscopy (IR). Osteoclasts are specialized multinucleated cells with bone resorbing activity. The induction of c-Fos and NFATc1 transcription factors by receptor activator of nuclear factor κB ligand (RANKL) is a key event in osteoclast differentiation. Isolated and identification compound 1 was investigated in vitro for their anti-osteoclastic activity. Compound 1 inhibited osteoclastic differentiation in a dose-dependent manner without cytotoxicity. In addition, the inhibitory mechanisms of compound 1 were investigated. Compound 1 induced a clear reduction in the expressions of NFATc1 and c-Fos upon RANKL stimulation. In summary, compound 1 from sea mustard showed antiosteoporotic potential.",

keywords = "Anti-osteoporosis, Fucosterol, Nuclear factor of activated T cells c1 (NFATc1), Receptor activator of nuclear factor κB ligand (RANKL), Sea mustard, Undaria pinnatifida",

author = "Bang, {Myun Ho} and Kim, {Hong Hee} and Lee, {Dae Young} and Han, {Min Woo} and Baek, {Yoon Su} and Chung, {Dae Kyun} and Baek, {Nam In}",

year = "2011",

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doi = "10.1007/s10068-011-0048-z",

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AU - Bang, Myun Ho

AU - Kim, Hong Hee

AU - Lee, Dae Young

AU - Han, Min Woo

AU - Baek, Yoon Su

AU - Chung, Dae Kyun

AU - Baek, Nam In

PY - 2011/4

Y1 - 2011/4

N2 - Samples of the edible seaweed, sea mustard (Undaria pinnatifida) were extracted with 80% aqueous methanol, and the concentrated extract was partitioned with ethyl acetate, n-butanol, and water, successively. Isolated fucosterol (1) from the ethyl acetate layers using repeated silica gel and octadecyl silica gel (C18, ODS-A) column chromatography and identified it using spectroscopic data including NMR, MS, and infrared spectroscopy (IR). Osteoclasts are specialized multinucleated cells with bone resorbing activity. The induction of c-Fos and NFATc1 transcription factors by receptor activator of nuclear factor κB ligand (RANKL) is a key event in osteoclast differentiation. Isolated and identification compound 1 was investigated in vitro for their anti-osteoclastic activity. Compound 1 inhibited osteoclastic differentiation in a dose-dependent manner without cytotoxicity. In addition, the inhibitory mechanisms of compound 1 were investigated. Compound 1 induced a clear reduction in the expressions of NFATc1 and c-Fos upon RANKL stimulation. In summary, compound 1 from sea mustard showed antiosteoporotic potential.

AB - Samples of the edible seaweed, sea mustard (Undaria pinnatifida) were extracted with 80% aqueous methanol, and the concentrated extract was partitioned with ethyl acetate, n-butanol, and water, successively. Isolated fucosterol (1) from the ethyl acetate layers using repeated silica gel and octadecyl silica gel (C18, ODS-A) column chromatography and identified it using spectroscopic data including NMR, MS, and infrared spectroscopy (IR). Osteoclasts are specialized multinucleated cells with bone resorbing activity. The induction of c-Fos and NFATc1 transcription factors by receptor activator of nuclear factor κB ligand (RANKL) is a key event in osteoclast differentiation. Isolated and identification compound 1 was investigated in vitro for their anti-osteoclastic activity. Compound 1 inhibited osteoclastic differentiation in a dose-dependent manner without cytotoxicity. In addition, the inhibitory mechanisms of compound 1 were investigated. Compound 1 induced a clear reduction in the expressions of NFATc1 and c-Fos upon RANKL stimulation. In summary, compound 1 from sea mustard showed antiosteoporotic potential.

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Anti-osteoporotic activities of fucosterol from sea mustard (Undaria pinnatifida)

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