Anti-osteoporotic activities of fucosterol from sea mustard (Undaria pinnatifida)

Myun Ho Bang; Hong Hee Kim; Dae Young Lee; Min Woo Han; Yoon Su Baek; Dae Kyun Chung; Nam In Baek

doi:10.1007/s10068-011-0048-z

Anti-osteoporotic activities of fucosterol from sea mustard (Undaria pinnatifida)

Myun Ho Bang
, Hong Hee Kim
, Dae Young Lee
, Min Woo Han
, Yoon Su Baek
, Dae Kyun Chung
, Nam In Baek

School of Life Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Samples of the edible seaweed, sea mustard (Undaria pinnatifida) were extracted with 80% aqueous methanol, and the concentrated extract was partitioned with ethyl acetate, n-butanol, and water, successively. Isolated fucosterol (1) from the ethyl acetate layers using repeated silica gel and octadecyl silica gel (C₁₈, ODS-A) column chromatography and identified it using spectroscopic data including NMR, MS, and infrared spectroscopy (IR). Osteoclasts are specialized multinucleated cells with bone resorbing activity. The induction of c-Fos and NFATc1 transcription factors by receptor activator of nuclear factor κB ligand (RANKL) is a key event in osteoclast differentiation. Isolated and identification compound 1 was investigated in vitro for their anti-osteoclastic activity. Compound 1 inhibited osteoclastic differentiation in a dose-dependent manner without cytotoxicity. In addition, the inhibitory mechanisms of compound 1 were investigated. Compound 1 induced a clear reduction in the expressions of NFATc1 and c-Fos upon RANKL stimulation. In summary, compound 1 from sea mustard showed antiosteoporotic potential.

Original language	English
Pages (from-to)	343-347
Number of pages	5
Journal	Food Science and Biotechnology
Volume	20
Issue number	2
DOIs	https://doi.org/10.1007/s10068-011-0048-z
State	Published - Apr 2011

Keywords

Anti-osteoporosis
Fucosterol
Nuclear factor of activated T cells c1 (NFATc1)
Receptor activator of nuclear factor κB ligand (RANKL)
Sea mustard
Undaria pinnatifida

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10.1007/s10068-011-0048-z

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title = "Anti-osteoporotic activities of fucosterol from sea mustard (Undaria pinnatifida)",

abstract = "Samples of the edible seaweed, sea mustard (Undaria pinnatifida) were extracted with 80\% aqueous methanol, and the concentrated extract was partitioned with ethyl acetate, n-butanol, and water, successively. Isolated fucosterol (1) from the ethyl acetate layers using repeated silica gel and octadecyl silica gel (C18, ODS-A) column chromatography and identified it using spectroscopic data including NMR, MS, and infrared spectroscopy (IR). Osteoclasts are specialized multinucleated cells with bone resorbing activity. The induction of c-Fos and NFATc1 transcription factors by receptor activator of nuclear factor κB ligand (RANKL) is a key event in osteoclast differentiation. Isolated and identification compound 1 was investigated in vitro for their anti-osteoclastic activity. Compound 1 inhibited osteoclastic differentiation in a dose-dependent manner without cytotoxicity. In addition, the inhibitory mechanisms of compound 1 were investigated. Compound 1 induced a clear reduction in the expressions of NFATc1 and c-Fos upon RANKL stimulation. In summary, compound 1 from sea mustard showed antiosteoporotic potential.",

keywords = "Anti-osteoporosis, Fucosterol, Nuclear factor of activated T cells c1 (NFATc1), Receptor activator of nuclear factor κB ligand (RANKL), Sea mustard, Undaria pinnatifida",

author = "Bang, \{Myun Ho\} and Kim, \{Hong Hee\} and Lee, \{Dae Young\} and Han, \{Min Woo\} and Baek, \{Yoon Su\} and Chung, \{Dae Kyun\} and Baek, \{Nam In\}",

year = "2011",

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doi = "10.1007/s10068-011-0048-z",

language = "English",

volume = "20",

pages = "343--347",

journal = "Food Science and Biotechnology",

issn = "1226-7708",

publisher = "The Korean Society of Food Science and Technology",

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T1 - Anti-osteoporotic activities of fucosterol from sea mustard (Undaria pinnatifida)

AU - Bang, Myun Ho

AU - Kim, Hong Hee

AU - Lee, Dae Young

AU - Han, Min Woo

AU - Baek, Yoon Su

AU - Chung, Dae Kyun

AU - Baek, Nam In

PY - 2011/4

Y1 - 2011/4

N2 - Samples of the edible seaweed, sea mustard (Undaria pinnatifida) were extracted with 80% aqueous methanol, and the concentrated extract was partitioned with ethyl acetate, n-butanol, and water, successively. Isolated fucosterol (1) from the ethyl acetate layers using repeated silica gel and octadecyl silica gel (C18, ODS-A) column chromatography and identified it using spectroscopic data including NMR, MS, and infrared spectroscopy (IR). Osteoclasts are specialized multinucleated cells with bone resorbing activity. The induction of c-Fos and NFATc1 transcription factors by receptor activator of nuclear factor κB ligand (RANKL) is a key event in osteoclast differentiation. Isolated and identification compound 1 was investigated in vitro for their anti-osteoclastic activity. Compound 1 inhibited osteoclastic differentiation in a dose-dependent manner without cytotoxicity. In addition, the inhibitory mechanisms of compound 1 were investigated. Compound 1 induced a clear reduction in the expressions of NFATc1 and c-Fos upon RANKL stimulation. In summary, compound 1 from sea mustard showed antiosteoporotic potential.

AB - Samples of the edible seaweed, sea mustard (Undaria pinnatifida) were extracted with 80% aqueous methanol, and the concentrated extract was partitioned with ethyl acetate, n-butanol, and water, successively. Isolated fucosterol (1) from the ethyl acetate layers using repeated silica gel and octadecyl silica gel (C18, ODS-A) column chromatography and identified it using spectroscopic data including NMR, MS, and infrared spectroscopy (IR). Osteoclasts are specialized multinucleated cells with bone resorbing activity. The induction of c-Fos and NFATc1 transcription factors by receptor activator of nuclear factor κB ligand (RANKL) is a key event in osteoclast differentiation. Isolated and identification compound 1 was investigated in vitro for their anti-osteoclastic activity. Compound 1 inhibited osteoclastic differentiation in a dose-dependent manner without cytotoxicity. In addition, the inhibitory mechanisms of compound 1 were investigated. Compound 1 induced a clear reduction in the expressions of NFATc1 and c-Fos upon RANKL stimulation. In summary, compound 1 from sea mustard showed antiosteoporotic potential.

KW - Anti-osteoporosis

KW - Fucosterol

KW - Nuclear factor of activated T cells c1 (NFATc1)

KW - Receptor activator of nuclear factor κB ligand (RANKL)

KW - Sea mustard

KW - Undaria pinnatifida

UR - https://www.scopus.com/pages/publications/79959714038

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DO - 10.1007/s10068-011-0048-z

M3 - Article

AN - SCOPUS:79959714038

SN - 1226-7708

VL - 20

SP - 343

EP - 347

JO - Food Science and Biotechnology

JF - Food Science and Biotechnology

IS - 2

ER -

Anti-osteoporotic activities of fucosterol from sea mustard (Undaria pinnatifida)

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