Abstract
Background: Recent research has shown that reactive oxygen species (ROS) play a signifcant role in the development and persistence of neuropathic pain through central sensitization via N-methyl-D-aspartate (NMDA) receptor activation. In the present study, we examined whether the intraperitoneal administration of vitamins C and E alone or together could alleviate mechanical allodynia in a chronic post-ischemia pain (CPIP) rat model. Methods: Vitamins C and E were administered intraperitoneally to 48 male Sprague Dawley rats once per day for 3 days before hindpaw ischemia-reperfusion (I/R) injury was induced. On the third day, the CPIP rat model was produced by inducing ischemia in the lef hindpaw by applying an O-ring for 3 h, followed by reperfusion. Tree days afer reperfu-sion, hindpaw mechanical allodynia was assessed by measuring the withdrawal response to von Frey flament stimulation. The rats were sacrificed immediately after behavioral testing to determine the phosphorylated NMDA receptor subunit 1 (pNR1) and extracellular-signal-regulated kinases (pERK) levels in the spinal cord. Results: When the antioxidant vitamins C and E were administered intraperitoneally to CPIP rats, I/R injury-induced mechanical allodynia was attenuated, and pNR1 and pERK levels were decreased in the rat spinal cord. Additionally, the co-administration of both vitamins had an increased antiallodynic effect. Conclusions: Te reduced phosphorylated NR1 and ERK levels indicate that vitamins C and E inhibit the modulation of spinal cord neuropathic pain processing. Co-administration of vitamins C and E had a greater antiallodynic effect.
Original language | English |
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Pages (from-to) | 442-448 |
Number of pages | 7 |
Journal | Korean Journal of Anesthesiology |
Volume | 65 |
Issue number | 5 |
DOIs | |
State | Published - Nov 2013 |
Keywords
- Antioxidants
- Complex regional pain syndromes
- Mitogen-activated protein kinases
- N-methyl-d-aspartate receptors
- Reperfusion injury
- Vitamins