Antifungal activity and mode of action of silver nano-particles on Candida albicans

Keuk Jun Kim; Woo Sang Sung; Bo Kyoung Suh; Seok Ki Moon; Jong Soo Choi; Jong Guk Kim; Dong Gun Lee

doi:10.1007/s10534-008-9159-2

Antifungal activity and mode of action of silver nano-particles on Candida albicans

Keuk Jun Kim, Woo Sang Sung, Bo Kyoung Suh, Seok Ki Moon, Jong Soo Choi, Jong Guk Kim, Dong Gun Lee

Research output: Contribution to journal › Article › peer-review

807 Scopus citations

Abstract

In this study, the antifungal effects of silver nano-particles (nano-Ag) and their mode of action were investigated. Nano-Ag showed antifungal effects on fungi tested with low hemolytic effects against human erythrocytes. To elucidate the antifungal mode of action of nano-Ag, flow cytometry analysis, a glucose-release test, transmission electron microscopy (TEM) and the change in membrane dynamics using 1,6-diphenyl-1,3,5-hexatriene (DPH), as a plasma membrane probe, were performed with Candida albicans. The results suggest nano-Ag may exert an antifungal activity by disrupting the structure of the cell membrane and inhibiting the normal budding process due to the destruction of the membrane integrity. The present study indicates nano-Ag has considerable antifungal activity, deserving further investigation for clinical applications.

Original language	English
Pages (from-to)	235-242
Number of pages	8
Journal	BioMetals
Volume	22
Issue number	2
DOIs	https://doi.org/10.1007/s10534-008-9159-2
State	Published - Apr 2009

Keywords

Antifungal activity
Candida albicans
Membrane disruption
Silver nano-particles

Access to Document

10.1007/s10534-008-9159-2

Cite this

@article{6a547230d55e448a87fe8f8ddc730245,

title = "Antifungal activity and mode of action of silver nano-particles on Candida albicans",

abstract = "In this study, the antifungal effects of silver nano-particles (nano-Ag) and their mode of action were investigated. Nano-Ag showed antifungal effects on fungi tested with low hemolytic effects against human erythrocytes. To elucidate the antifungal mode of action of nano-Ag, flow cytometry analysis, a glucose-release test, transmission electron microscopy (TEM) and the change in membrane dynamics using 1,6-diphenyl-1,3,5-hexatriene (DPH), as a plasma membrane probe, were performed with Candida albicans. The results suggest nano-Ag may exert an antifungal activity by disrupting the structure of the cell membrane and inhibiting the normal budding process due to the destruction of the membrane integrity. The present study indicates nano-Ag has considerable antifungal activity, deserving further investigation for clinical applications.",

keywords = "Antifungal activity, Candida albicans, Membrane disruption, Silver nano-particles",

author = "Kim, \{Keuk Jun\} and Sung, \{Woo Sang\} and Suh, \{Bo Kyoung\} and Moon, \{Seok Ki\} and Choi, \{Jong Soo\} and Kim, \{Jong Guk\} and Lee, \{Dong Gun\}",

year = "2009",

month = apr,

doi = "10.1007/s10534-008-9159-2",

language = "English",

volume = "22",

pages = "235--242",

journal = "BioMetals",

issn = "0966-0844",

publisher = "Springer Science and Business Media B.V.",

number = "2",

}

TY - JOUR

T1 - Antifungal activity and mode of action of silver nano-particles on Candida albicans

AU - Kim, Keuk Jun

AU - Sung, Woo Sang

AU - Suh, Bo Kyoung

AU - Moon, Seok Ki

AU - Choi, Jong Soo

AU - Kim, Jong Guk

AU - Lee, Dong Gun

PY - 2009/4

Y1 - 2009/4

N2 - In this study, the antifungal effects of silver nano-particles (nano-Ag) and their mode of action were investigated. Nano-Ag showed antifungal effects on fungi tested with low hemolytic effects against human erythrocytes. To elucidate the antifungal mode of action of nano-Ag, flow cytometry analysis, a glucose-release test, transmission electron microscopy (TEM) and the change in membrane dynamics using 1,6-diphenyl-1,3,5-hexatriene (DPH), as a plasma membrane probe, were performed with Candida albicans. The results suggest nano-Ag may exert an antifungal activity by disrupting the structure of the cell membrane and inhibiting the normal budding process due to the destruction of the membrane integrity. The present study indicates nano-Ag has considerable antifungal activity, deserving further investigation for clinical applications.

AB - In this study, the antifungal effects of silver nano-particles (nano-Ag) and their mode of action were investigated. Nano-Ag showed antifungal effects on fungi tested with low hemolytic effects against human erythrocytes. To elucidate the antifungal mode of action of nano-Ag, flow cytometry analysis, a glucose-release test, transmission electron microscopy (TEM) and the change in membrane dynamics using 1,6-diphenyl-1,3,5-hexatriene (DPH), as a plasma membrane probe, were performed with Candida albicans. The results suggest nano-Ag may exert an antifungal activity by disrupting the structure of the cell membrane and inhibiting the normal budding process due to the destruction of the membrane integrity. The present study indicates nano-Ag has considerable antifungal activity, deserving further investigation for clinical applications.

KW - Antifungal activity

KW - Candida albicans

KW - Membrane disruption

KW - Silver nano-particles

UR - https://www.scopus.com/pages/publications/63149166892

U2 - 10.1007/s10534-008-9159-2

DO - 10.1007/s10534-008-9159-2

M3 - Article

C2 - 18769871

AN - SCOPUS:63149166892

SN - 0966-0844

VL - 22

SP - 235

EP - 242

JO - BioMetals

JF - BioMetals

IS - 2

ER -

Antifungal activity and mode of action of silver nano-particles on Candida albicans

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this