Antifungal mechanism of a cysteine-rich antimicrobial peptide, Ib-AMP1, from Impatiens balsamina against Candida albicans

Dong Gun Lee; Song Yub Shin; Dae Hee Kim; Moo Yeol Seo; Joo Hyun Kang; Younghoon Lee; Kil Lyong Kim; Kyung Soo Hahm

doi:10.1023/A:1005636610512

Antifungal mechanism of a cysteine-rich antimicrobial peptide, Ib-AMP1, from Impatiens balsamina against Candida albicans

Dong Gun Lee, Song Yub Shin, Dae Hee Kim, Moo Yeol Seo, Joo Hyun Kang, Younghoon Lee, Kil Lyong Kim, Kyung Soo Hahm

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39 Scopus citations

Abstract

The antifungal mechanism of a 20-mer peptide, Ib-AMP1, derived from Impatiens balsamina was investigated. The oxidized (disulfide bridged) Ib- AMP1 showed a 4-fold increase in antifungal activity against Aspergillus flavus and Candida albicans than reduced (non-disulfide bridged) Ib-AMP1. Ib- AMP1 had very low activity for phospholipid disruption when compared with cecropin A(1-8)-magainin 2(1-12), a α-helical amphiphatic, antimicrobial peptide. Confocal microscopy showed that Ib-AMP1 binds on cell surface or penetrates into cell membranes. These results suggested that Ib-AMP1 may manifest its antifungal activity against Candida albicans by inhibiting a distinct cellular process rather than ion channel or pore formation in cell membrane.

Original language	English
Pages (from-to)	1047-1050
Number of pages	4
Journal	Biotechnology Letters
Volume	21
Issue number	12
DOIs	https://doi.org/10.1023/A:1005636610512
State	Published - 1999

Keywords

Antifungal mechanism
Candida albicans
Confocal microscopy
Ib-AMP1

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title = "Antifungal mechanism of a cysteine-rich antimicrobial peptide, Ib-AMP1, from Impatiens balsamina against Candida albicans",

abstract = "The antifungal mechanism of a 20-mer peptide, Ib-AMP1, derived from Impatiens balsamina was investigated. The oxidized (disulfide bridged) Ib- AMP1 showed a 4-fold increase in antifungal activity against Aspergillus flavus and Candida albicans than reduced (non-disulfide bridged) Ib-AMP1. Ib- AMP1 had very low activity for phospholipid disruption when compared with cecropin A(1-8)-magainin 2(1-12), a α-helical amphiphatic, antimicrobial peptide. Confocal microscopy showed that Ib-AMP1 binds on cell surface or penetrates into cell membranes. These results suggested that Ib-AMP1 may manifest its antifungal activity against Candida albicans by inhibiting a distinct cellular process rather than ion channel or pore formation in cell membrane.",

keywords = "Antifungal mechanism, Candida albicans, Confocal microscopy, Ib-AMP1",

author = "Lee, {Dong Gun} and Shin, {Song Yub} and Kim, {Dae Hee} and Seo, {Moo Yeol} and Kang, {Joo Hyun} and Younghoon Lee and Kim, {Kil Lyong} and Hahm, {Kyung Soo}",

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doi = "10.1023/A:1005636610512",

language = "English",

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journal = "Biotechnology Letters",

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T1 - Antifungal mechanism of a cysteine-rich antimicrobial peptide, Ib-AMP1, from Impatiens balsamina against Candida albicans

AU - Lee, Dong Gun

AU - Shin, Song Yub

AU - Kim, Dae Hee

AU - Seo, Moo Yeol

AU - Kang, Joo Hyun

AU - Lee, Younghoon

AU - Kim, Kil Lyong

AU - Hahm, Kyung Soo

PY - 1999

Y1 - 1999

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AB - The antifungal mechanism of a 20-mer peptide, Ib-AMP1, derived from Impatiens balsamina was investigated. The oxidized (disulfide bridged) Ib- AMP1 showed a 4-fold increase in antifungal activity against Aspergillus flavus and Candida albicans than reduced (non-disulfide bridged) Ib-AMP1. Ib- AMP1 had very low activity for phospholipid disruption when compared with cecropin A(1-8)-magainin 2(1-12), a α-helical amphiphatic, antimicrobial peptide. Confocal microscopy showed that Ib-AMP1 binds on cell surface or penetrates into cell membranes. These results suggested that Ib-AMP1 may manifest its antifungal activity against Candida albicans by inhibiting a distinct cellular process rather than ion channel or pore formation in cell membrane.

KW - Antifungal mechanism

KW - Candida albicans

KW - Confocal microscopy

KW - Ib-AMP1

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DO - 10.1023/A:1005636610512

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JO - Biotechnology Letters

JF - Biotechnology Letters

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ER -

Antifungal mechanism of a cysteine-rich antimicrobial peptide, Ib-AMP1, from Impatiens balsamina against Candida albicans

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Keywords

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