TY - JOUR
T1 - Antifungal mechanism of antifungal peptide derived from cecropin A(1- 8)-melittin(1-12) hybrid against Aspergillus fumigatus
AU - Lee, Dong Gun
AU - Jin, Zhe Zhu
AU - Maeng, Cheol Young
AU - Shin, Song Yub
AU - Seo, Moo Yeol
AU - Kim, Kil Lyong
AU - Hahm, Kyung Soo
PY - 1999/4
Y1 - 1999/4
N2 - The antifungal mechanism of the antifungal peptide against Aspergillus fumigatus, K18, 19CA-(1-8)-ME(1-12), derived from cecropin A(1-8)- melittin(1-12) was investigated by confocal laser scanning microscopy, cell wall regeneration, ATPase activity inhibition, and released potassium ion. By confocal laser scanning microscopy, K18, 19-CA(1-8)-ME(1-12) was detected on the surface of A. fumigatus, while cecropin A used as a negative control peptide was not detected. The protoplast of A. fumigatus treated with K18, 19-CA(1-8)-ME(1-12) failed to regenerate the fungal cell walls. Compared with cecropin A, the amount of potassium ion released by K18, 19-CA(1-8)-ME(1-12) was increased. Furthermore, K18, 19-CA(1-8)-ME(1- 12) inhibited the ATPase activity on the plasma membrane. These results suggested that K18, 19-CA(1-8)-ME (1-12) acts on the plasma membrane of A. fumigatus and its antifungal action is due to the ion channel or pore formation on the plasma membrane.
AB - The antifungal mechanism of the antifungal peptide against Aspergillus fumigatus, K18, 19CA-(1-8)-ME(1-12), derived from cecropin A(1-8)- melittin(1-12) was investigated by confocal laser scanning microscopy, cell wall regeneration, ATPase activity inhibition, and released potassium ion. By confocal laser scanning microscopy, K18, 19-CA(1-8)-ME(1-12) was detected on the surface of A. fumigatus, while cecropin A used as a negative control peptide was not detected. The protoplast of A. fumigatus treated with K18, 19-CA(1-8)-ME(1-12) failed to regenerate the fungal cell walls. Compared with cecropin A, the amount of potassium ion released by K18, 19-CA(1-8)-ME(1-12) was increased. Furthermore, K18, 19-CA(1-8)-ME(1- 12) inhibited the ATPase activity on the plasma membrane. These results suggested that K18, 19-CA(1-8)-ME (1-12) acts on the plasma membrane of A. fumigatus and its antifungal action is due to the ion channel or pore formation on the plasma membrane.
KW - Aspergillus fumigatus
KW - Cecropin A
KW - Melittin
UR - http://www.scopus.com/inward/record.url?scp=0344654684&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0344654684
SN - 1017-7825
VL - 9
SP - 168
EP - 172
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 2
ER -