TY - JOUR
T1 - Antimicrobial and plant growth-promoting activities of bacterial endophytes isolated from Calotropis procera (Ait.) W.T. Aiton
AU - Hamayun, Muhammad
AU - Khan, Nasir
AU - Khan, Muhammad Nauman
AU - Qadir, Muhammad
AU - Hussain, Anwar
AU - Iqbal, Amjad
AU - Khan, Sumera Afzal
AU - Ur Rehman, Khushnood
AU - Lee, In Jung
N1 - Publisher Copyright:
© 2021 Centro Regional de Invest. Cientif. y Tecn.. All rights reserved.
PY - 2021/2
Y1 - 2021/2
N2 - Bacterial endophytes are beneficial to their hosts as they can fix nitrogen in the soil and make it available to the host. Endophytic bacteria also secrete plant growth-promoting hormones to support their host plants under normal as well as stress conditions. The current study aimed to isolate endophytic bacteria from different parts of Calotropis procera, i.e., roots, stem and leaves of Calotropis procera (Ait.) W.T. Aiton. Plants were collected from the Lundkhwar, district Mardan. A total of 12 bacterial strains, i.e., six from roots, three from the stem and three from the leaves were isolated. The strains were screened for their growth-promoting activity in rice plants because rice shows a quick and easy response to the bioactive compounds present in the culture filtrate (CF) of the potent endophytic strains. The rice plants were cultivated in pots containing 30 mL of 0.8% w/v water-agar medium. The pots were placed in a growth chamber, operated at 28 ± 0.3°C for 14 h (day); and 25 ± 0.3°C for 10 h (night), at 70% relative-humidity. Among the isolated strains, R1, S1, S3, L1, R5 and R6 showed visible growth promotion in rice plants. The biochemical analysis revealed that the strains were able to produce indole acetic acid (IAA) and flavonoids in higher quantities. Moreover, the strains also produced bioactive compounds that inhibited the growth of Escherichia coli and Aspergillus flavus using the well diffusion method. From the results, it was concluded that these strains can secrete potent compounds that can promote the host plant growth and inhibit the growth of pathogenic microorganisms and, therefore, can be used as bio-fertilizer and bio-control agents.
AB - Bacterial endophytes are beneficial to their hosts as they can fix nitrogen in the soil and make it available to the host. Endophytic bacteria also secrete plant growth-promoting hormones to support their host plants under normal as well as stress conditions. The current study aimed to isolate endophytic bacteria from different parts of Calotropis procera, i.e., roots, stem and leaves of Calotropis procera (Ait.) W.T. Aiton. Plants were collected from the Lundkhwar, district Mardan. A total of 12 bacterial strains, i.e., six from roots, three from the stem and three from the leaves were isolated. The strains were screened for their growth-promoting activity in rice plants because rice shows a quick and easy response to the bioactive compounds present in the culture filtrate (CF) of the potent endophytic strains. The rice plants were cultivated in pots containing 30 mL of 0.8% w/v water-agar medium. The pots were placed in a growth chamber, operated at 28 ± 0.3°C for 14 h (day); and 25 ± 0.3°C for 10 h (night), at 70% relative-humidity. Among the isolated strains, R1, S1, S3, L1, R5 and R6 showed visible growth promotion in rice plants. The biochemical analysis revealed that the strains were able to produce indole acetic acid (IAA) and flavonoids in higher quantities. Moreover, the strains also produced bioactive compounds that inhibited the growth of Escherichia coli and Aspergillus flavus using the well diffusion method. From the results, it was concluded that these strains can secrete potent compounds that can promote the host plant growth and inhibit the growth of pathogenic microorganisms and, therefore, can be used as bio-fertilizer and bio-control agents.
KW - Antimicrobial activity
KW - Endophytic bacteria
KW - Growth promotion
KW - Plant-microbe interaction
UR - http://www.scopus.com/inward/record.url?scp=85103178184&partnerID=8YFLogxK
U2 - 10.32604/BIOCELL.2021.013907
DO - 10.32604/BIOCELL.2021.013907
M3 - Article
AN - SCOPUS:85103178184
SN - 0327-9545
VL - 45
SP - 363
EP - 369
JO - Biocell
JF - Biocell
IS - 2
ER -