Antimorphic PV.1 Causes secondary axis by inducing ectopic organizer

Yoo Seok Hwang; Jeong Jae Seo; Sang Wook Cha; Mae Ja Park; Hyun Shik Lee; Sung Young Lee; Jaebong Kim; Hsiang fu Kung; Dong Hyun Roh

doi:10.1006/bbrc.2002.6740

Antimorphic PV.1 Causes secondary axis by inducing ectopic organizer

Yoo Seok Hwang, Jeong Jae Seo, Sang Wook Cha, Mae Ja Park, Hyun Shik Lee, Sung Young Lee, Jaebong Kim, Hsiang fu Kung, Dong Hyun Roh

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Xenopus homeobox gene, PV.1 ventralizes activin-induced dorsal mesoderm and inhibits neuralization of ectoderm in animal cap when overexpressed. Here we generated PV.1/engrailed fusion construct (N-PV1-EnR) to perform loss-of-function study for this transcription factor. N-PV1-EnR showed an extremely antimorphic effect, causing a partial secondary embryonic axis when expressed at ventral marginal zone of blastula. In ventral marginal zone cells, this chimeric protein induced organizer genes and suppressed ventral markers mimicking those effects reported for dominant negative BMP-4 receptor (DNBR). Moreover, N-PV1-EnR rescued the ventralized embryos caused by the ectopic dorsal expression of PV.1 but not by that of Xvent-2. These results suggested that PV.1 functions at downstream of BMP-4 as a ventralizing effector which acts separately from Xvent-2 and the dominant negative effect gained by this specific mutant is applicable for the further studies of BMP-4 downstream pathway.

Original language	English
Pages (from-to)	1081-1086
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	292
Issue number	4
DOIs	https://doi.org/10.1006/bbrc.2002.6740
State	Published - 2002

Keywords

Antimorphic PV.1
BMP-4
Dorsoventral axis
Ectopic organizer
Homeobox
Loss-of-function
PV.1
Secondary axis
Ventralization
Xenopus laevis embryo

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title = "Antimorphic PV.1 Causes secondary axis by inducing ectopic organizer",

abstract = "Xenopus homeobox gene, PV.1 ventralizes activin-induced dorsal mesoderm and inhibits neuralization of ectoderm in animal cap when overexpressed. Here we generated PV.1/engrailed fusion construct (N-PV1-EnR) to perform loss-of-function study for this transcription factor. N-PV1-EnR showed an extremely antimorphic effect, causing a partial secondary embryonic axis when expressed at ventral marginal zone of blastula. In ventral marginal zone cells, this chimeric protein induced organizer genes and suppressed ventral markers mimicking those effects reported for dominant negative BMP-4 receptor (DNBR). Moreover, N-PV1-EnR rescued the ventralized embryos caused by the ectopic dorsal expression of PV.1 but not by that of Xvent-2. These results suggested that PV.1 functions at downstream of BMP-4 as a ventralizing effector which acts separately from Xvent-2 and the dominant negative effect gained by this specific mutant is applicable for the further studies of BMP-4 downstream pathway.",

keywords = "Antimorphic PV.1, BMP-4, Dorsoventral axis, Ectopic organizer, Homeobox, Loss-of-function, PV.1, Secondary axis, Ventralization, Xenopus laevis embryo",

author = "Hwang, {Yoo Seok} and Seo, {Jeong Jae} and Cha, {Sang Wook} and {Ja Park}, Mae and Lee, {Hyun Shik} and Lee, {Sung Young} and Jaebong Kim and Kung, {Hsiang fu} and Roh, {Dong Hyun}",

year = "2002",

doi = "10.1006/bbrc.2002.6740",

language = "English",

volume = "292",

pages = "1081--1086",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Antimorphic PV.1 Causes secondary axis by inducing ectopic organizer

AU - Hwang, Yoo Seok

AU - Seo, Jeong Jae

AU - Cha, Sang Wook

AU - Ja Park, Mae

AU - Lee, Hyun Shik

AU - Lee, Sung Young

AU - Kim, Jaebong

AU - Kung, Hsiang fu

AU - Roh, Dong Hyun

PY - 2002

Y1 - 2002

N2 - Xenopus homeobox gene, PV.1 ventralizes activin-induced dorsal mesoderm and inhibits neuralization of ectoderm in animal cap when overexpressed. Here we generated PV.1/engrailed fusion construct (N-PV1-EnR) to perform loss-of-function study for this transcription factor. N-PV1-EnR showed an extremely antimorphic effect, causing a partial secondary embryonic axis when expressed at ventral marginal zone of blastula. In ventral marginal zone cells, this chimeric protein induced organizer genes and suppressed ventral markers mimicking those effects reported for dominant negative BMP-4 receptor (DNBR). Moreover, N-PV1-EnR rescued the ventralized embryos caused by the ectopic dorsal expression of PV.1 but not by that of Xvent-2. These results suggested that PV.1 functions at downstream of BMP-4 as a ventralizing effector which acts separately from Xvent-2 and the dominant negative effect gained by this specific mutant is applicable for the further studies of BMP-4 downstream pathway.

AB - Xenopus homeobox gene, PV.1 ventralizes activin-induced dorsal mesoderm and inhibits neuralization of ectoderm in animal cap when overexpressed. Here we generated PV.1/engrailed fusion construct (N-PV1-EnR) to perform loss-of-function study for this transcription factor. N-PV1-EnR showed an extremely antimorphic effect, causing a partial secondary embryonic axis when expressed at ventral marginal zone of blastula. In ventral marginal zone cells, this chimeric protein induced organizer genes and suppressed ventral markers mimicking those effects reported for dominant negative BMP-4 receptor (DNBR). Moreover, N-PV1-EnR rescued the ventralized embryos caused by the ectopic dorsal expression of PV.1 but not by that of Xvent-2. These results suggested that PV.1 functions at downstream of BMP-4 as a ventralizing effector which acts separately from Xvent-2 and the dominant negative effect gained by this specific mutant is applicable for the further studies of BMP-4 downstream pathway.

KW - Antimorphic PV.1

KW - BMP-4

KW - Dorsoventral axis

KW - Ectopic organizer

KW - Homeobox

KW - Loss-of-function

KW - PV.1

KW - Secondary axis

KW - Ventralization

KW - Xenopus laevis embryo

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M3 - Article

C2 - 11944926

AN - SCOPUS:0036280526

SN - 0006-291X

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EP - 1086

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Antimorphic PV.1 Causes secondary axis by inducing ectopic organizer

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