Antioxidant effect of wheat germ extracts and their antilipidemic effect in palmitic acid-induced steatosis in hepg2 and 3t3-l1 cells

Humna Liaqat, Kyeong Jin Kim, Soo Yeon Park, Sung Keun Jung, Sung Hee Park, Seokwon Lim, Ji Yeon Kim

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Wheat germ (WG) is a by-product of wheat milling and comprises many bioactive com-pounds. This study aimed to compare the antioxidant and antilipidemic effects of different WG extracts (WGEs) by analyzing candidate bioactive compounds such as carotenoids, tocopherols, γ-oryzanol, and biogenic amines by reversed-phase high-performance liquid chromatography. An-tioxidant activity was determined using the ABTS, DPPH, and FRAP assays. The antilipidemic effect was evaluated in palmitic acid-induced steatosis in HepG2 hepatocytes and 3T3-L1 adipocytes. Cellular lipid accumulation was assessed by Oil Red O staining and a cellular triglyceride content assay. All analyzed WGEs showed significant antioxidant potential, although some bioactive com-pounds, such as carotenoids, tocopherols, and γ-oryzanol, were the highest in the ethanol extract. Correlation analysis revealed the antioxidant potential of all identified biogenic amines except for spermidine. Ethanol and n-hexane extracts significantly inhibited cellular lipid accumulation in cell models. These results suggest that WGEs exhibit promising antioxidant potential, with a variety of bioactive compounds. Collectively, the findings of this study suggest that bioactive compounds in WGEs attenuate plasma lipid and oxidation levels. In conclusion, WG can be used as a natural antioxidant and nutraceutical using appropriate solvents and extraction methods.

Original languageEnglish
Article number1061
JournalFoods
Volume10
Issue number5
DOIs
StatePublished - May 2021

Keywords

  • Antilipidemic effect
  • Antioxidants
  • Bioactive compounds
  • Wheat germ extracts

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