Antithrombotic activities of oroxylin A in vitro and in vivo

Sae Kwang Ku, In Chul Lee, Jong Sup Bae

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Here, the anticoagulant activities of oroxylin A (OroA), a major component of Scutellaria baicalensis Georgi, were examined by monitoring activated partial thromboplastin time (aPTT), prothrombin time (PT), and the activities of cell-based thrombin and activated factor X (FXa). Furthermore, the effects of OroA on the expressions of plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) were tested in tumor necrosis factor (TNF)-α activated human umbilical vein endothelial cells (HUVECs). Treatment with OroA resulted in prolonged aPTT and PT and inhibition of the activities of thrombin and FXa, and OroA inhibited production of thrombin and FXa in HUVECs. And OroA inhibited thrombin-catalyzed fibrin polymerization and platelet aggregation. In accordance with these anticoagulant activities, OroA elicited anticoagulant effects in mouse. In addition, treatment of OroA resulted in the inhibition of TNF-α-induced production of PAI-1, and treatment with OroA resulted in the significant reduction of the PAI-1 to t-PA ratio. Collectively, OroA possess antithrombotic activities and offer bases for development of a novel anticoagulant.

Original languageEnglish
Pages (from-to)679-686
Number of pages8
JournalArchives of Pharmacal Research
Volume37
Issue number5
DOIs
StatePublished - May 2014

Keywords

  • Coagulation cascade
  • Endothelium
  • Fibrinolysis
  • Oroxylin A

Fingerprint

Dive into the research topics of 'Antithrombotic activities of oroxylin A in vitro and in vivo'. Together they form a unique fingerprint.

Cite this