Application of an enzyme encapsulated metal-organic framework composite for convenient sensing and degradation of methyl parathion

Jyotsana Mehta; Sarita Dhaka; Neha Bhardwaj; Ashok K. Paul; Siddavattam Dayananda; Sung Eun Lee; Ki Hyun Kim; Akash Deep

doi:10.1016/j.snb.2019.03.116

Application of an enzyme encapsulated metal-organic framework composite for convenient sensing and degradation of methyl parathion

Jyotsana Mehta, Sarita Dhaka, Neha Bhardwaj, Ashok K. Paul, Siddavattam Dayananda, Sung Eun Lee, Ki Hyun Kim, Akash Deep

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

Terbium-BTC (BTC = 1,2,4-Benzenetricarboxylic acid) metal-organic framework (MOF) has been employed for in-situ encapsulation of hexahistidine-tagged organophosphate hydrolase (OPH^6His) enzyme. The enzymatic activity and stability of OPH^6His were found remarkably enhanced after its encapsulation within the MOF. The potential of this enzyme-MOF composite (OPH^6His@Tb-BTC) has been explored as a dual platform to simultaneously detect organophosphate pesticides (OPPs) and catalyze their degradation. The OPH^6His@Tb-BTC exhibited improved enzymatic activity (by approximately 30%) and reusability (at least five cycles). It is demonstrated to be a highly sensitive and simple optical bioprobe for the detection of an OPP candidate, methyl parathion with a low limit of detection (2.6 nM). A less toxic metabolite was formed through catalytic conversion of methyl parathion (i.e., upon its incubation with OPH^6His@Tb-BTC), further supporting the promising role of this composite in the remediation of pesticide-contaminated environments.

Original language	English
Pages (from-to)	267-274
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	290
DOIs	https://doi.org/10.1016/j.snb.2019.03.116
State	Published - 1 Jul 2019

Keywords

Degradation
MOF
Methyl parathion
Organophosphate hydrolase
Organophosphates
Sensing

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10.1016/j.snb.2019.03.116

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title = "Application of an enzyme encapsulated metal-organic framework composite for convenient sensing and degradation of methyl parathion",

abstract = "Terbium-BTC (BTC = 1,2,4-Benzenetricarboxylic acid) metal-organic framework (MOF) has been employed for in-situ encapsulation of hexahistidine-tagged organophosphate hydrolase (OPH6His) enzyme. The enzymatic activity and stability of OPH6His were found remarkably enhanced after its encapsulation within the MOF. The potential of this enzyme-MOF composite (OPH6His@Tb-BTC) has been explored as a dual platform to simultaneously detect organophosphate pesticides (OPPs) and catalyze their degradation. The OPH6His@Tb-BTC exhibited improved enzymatic activity (by approximately 30\%) and reusability (at least five cycles). It is demonstrated to be a highly sensitive and simple optical bioprobe for the detection of an OPP candidate, methyl parathion with a low limit of detection (2.6 nM). A less toxic metabolite was formed through catalytic conversion of methyl parathion (i.e., upon its incubation with OPH6His@Tb-BTC), further supporting the promising role of this composite in the remediation of pesticide-contaminated environments.",

keywords = "Degradation, MOF, Methyl parathion, Organophosphate hydrolase, Organophosphates, Sensing",

author = "Jyotsana Mehta and Sarita Dhaka and Neha Bhardwaj and Paul, \{Ashok K.\} and Siddavattam Dayananda and Lee, \{Sung Eun\} and Kim, \{Ki Hyun\} and Akash Deep",

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T1 - Application of an enzyme encapsulated metal-organic framework composite for convenient sensing and degradation of methyl parathion

AU - Mehta, Jyotsana

AU - Dhaka, Sarita

AU - Bhardwaj, Neha

AU - Paul, Ashok K.

AU - Dayananda, Siddavattam

AU - Lee, Sung Eun

AU - Kim, Ki Hyun

AU - Deep, Akash

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Terbium-BTC (BTC = 1,2,4-Benzenetricarboxylic acid) metal-organic framework (MOF) has been employed for in-situ encapsulation of hexahistidine-tagged organophosphate hydrolase (OPH6His) enzyme. The enzymatic activity and stability of OPH6His were found remarkably enhanced after its encapsulation within the MOF. The potential of this enzyme-MOF composite (OPH6His@Tb-BTC) has been explored as a dual platform to simultaneously detect organophosphate pesticides (OPPs) and catalyze their degradation. The OPH6His@Tb-BTC exhibited improved enzymatic activity (by approximately 30%) and reusability (at least five cycles). It is demonstrated to be a highly sensitive and simple optical bioprobe for the detection of an OPP candidate, methyl parathion with a low limit of detection (2.6 nM). A less toxic metabolite was formed through catalytic conversion of methyl parathion (i.e., upon its incubation with OPH6His@Tb-BTC), further supporting the promising role of this composite in the remediation of pesticide-contaminated environments.

AB - Terbium-BTC (BTC = 1,2,4-Benzenetricarboxylic acid) metal-organic framework (MOF) has been employed for in-situ encapsulation of hexahistidine-tagged organophosphate hydrolase (OPH6His) enzyme. The enzymatic activity and stability of OPH6His were found remarkably enhanced after its encapsulation within the MOF. The potential of this enzyme-MOF composite (OPH6His@Tb-BTC) has been explored as a dual platform to simultaneously detect organophosphate pesticides (OPPs) and catalyze their degradation. The OPH6His@Tb-BTC exhibited improved enzymatic activity (by approximately 30%) and reusability (at least five cycles). It is demonstrated to be a highly sensitive and simple optical bioprobe for the detection of an OPP candidate, methyl parathion with a low limit of detection (2.6 nM). A less toxic metabolite was formed through catalytic conversion of methyl parathion (i.e., upon its incubation with OPH6His@Tb-BTC), further supporting the promising role of this composite in the remediation of pesticide-contaminated environments.

KW - Degradation

KW - MOF

KW - Methyl parathion

KW - Organophosphate hydrolase

KW - Organophosphates

KW - Sensing

UR - https://www.scopus.com/pages/publications/85063896966

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DO - 10.1016/j.snb.2019.03.116

M3 - Article

AN - SCOPUS:85063896966

SN - 0925-4005

VL - 290

SP - 267

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JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

Application of an enzyme encapsulated metal-organic framework composite for convenient sensing and degradation of methyl parathion

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Keywords

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