Application of phage display for ligand peptidomics to identify peptide ligands binding to AQP2-expressing membrane fractions

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

In vitro phage display represents an emerging and innovative technology for the rapid isolation of high-affinity peptide ligands. Phage display technologies using phages comprising a vast library of peptides have become fundamental to the isolation of high-affinity binding ligands for diagnostic and therapeutic applications, e.g., ligand proteomics, discovery of novel protein-protein interactions, antibody engineering, targeted delivery of therapeutic agents, and development of imaging probes. This chapter describes the procedures for phage display selection of peptide ligands that selectively bind to aquaporin-2-expressing membrane fractions of rat kidney.

Original languageEnglish
Title of host publicationThe Low Molecular Weight Proteome
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages181-189
Number of pages9
ISBN (Print)9781461471677
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1023
ISSN (Print)1064-3745

Keywords

  • Aquaporin
  • Collecting duct
  • In vitro phage display
  • Ligand peptidomics
  • Vasopressin

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